Overview

  • Product name

  • Description

    Rabbit polyclonal to MICA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A 16 amino acid peptide from near the carboxy terminus of human MICA.

  • Positive control

    • A549 cell lysate and A-20 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 43 kDa).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function

    Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
  • Tissue specificity

    Widely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles. Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T cells.
  • Involvement in disease

    Note=Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma.
    Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1) [MIM:177900]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
    Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities

    Belongs to the MHC class I family. MIC subfamily.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.
    Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.
  • Cellular localization

    Cell membrane. Cytoplasm. Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • HLA class I antigen antibody
    • FLJ36918 antibody
    • FLJ60820 antibody
    • MGC111087 antibody
    • MGC21250 antibody
    • MHC class I chain related gene A protein antibody
    • MHC class I chain related protein A antibody
    • MHC class I chain related protein A HLA B HLA C antibody
    • MHC class I polypeptide related sequence A antibody
    • MHC class I polypeptide-related sequence A antibody
    • MHC class I related protein antibody
    • MIC A antibody
    • MIC-A antibody
    • micA antibody
    • MICA_HUMAN antibody
    • OTTHUMP00000029088 antibody
    • OTTHUMP00000044528 antibody
    • OTTHUMP00000165170 antibody
    • OTTHUMP00000165172 antibody
    • PERB11.1 antibody
    • Stress inducible class I homolog antibody
    see all

Images

  • Lane 1 : Anti-MICA antibody (ab62540) at 0.5 µg/ml
    Lane 2 : Anti-MICA antibody (ab62540) at 1 µg/ml
    Lane 3 : Anti-MICA antibody (ab62540) at 2 µg/ml

    All lanes : A-20 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 43 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?

  • ab62540 at 10µg/ml staining MICA in human colon tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human atheromatous aorta, staining MICA with ab62540.
  • Immunofluorescence of MICA in Human Colon cells using ab62540 at 20 ug/ml.

References

This product has been referenced in:

  • Kerekes D  et al. CD56+ immune cell infiltration and MICA are decreased in breast lobules with fibrocystic changes. Breast Cancer Res Treat 167:649-658 (2018). IHC-P ; Human . Read more (PubMed: 29090365) »
  • Brandetti E  et al. MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma. Oncoimmunology 6:e1316439 (2017). Read more (PubMed: 28680748) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Leukemia bone marrow)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Both Tris-EDTA and Citrate-Sodium
Specification
Leukemia bone marrow
Blocking step
(agent) for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 03 2015

Application
Flow Cytometry
Sample
Human Cell (Ovarian Cancer Cell Line TOV21G)
Permeabilization
No
Gating Strategy
live cells
Specification
Ovarian Cancer Cell Line TOV21G
Preparation
Cell harvesting/tissue preparation method: Typsin
Sample buffer: 1% BSA in PBS with 0.05% sodium azide
Fixation
no fixation

Abcam user community

Verified customer

Submitted Jun 15 2015

Question
Answer

Thank you for your reply.

We currently have two primary antibody / blocking peptide pairs to MICA for use in IHC-P:

Antibody ab62540 reacts with human and mouse, matching peptide: ab109170

Antibody ab93170 reacts with human, matching peptide: ab109171

Unfortunately, neither of these antibodies have been tested for cross-reactivity with MICB. I hope this helps, please let me know if you need any additional information.

Read More

Answer

Thank you for contacting Abcam regarding ab62540.

Regarding the method of antigen retrieval, this product was validated using heat mediated retieval. The protocol is as follows:


- Soak the slides in 10mM citrate buffer and cover with a lid.

- Microwave until the liquid boils, about 1-5 minutes.

- Remove from heat and let it stand at room temperature for 20 minutes

I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More

Answer

Thank you for your enquiry. Here are the answers to your questions. 1) ab93170 The immunogen was selected against the sequence of Q29983.  This MICA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75~105 amino acids from the center region of human MICA. It can potentially recognize the majority of the forms unless any changes occur within the region of 75-105 amino acids.  The immunogen shares 73% homology with MICB.  Unfortunately, there is a slight possibility that it could also detect MICB.  2) ab62540 We were only able to identify two isoforms of MICA.  The antigen is located within the last 30aa of the longest isoform and will thus not detect the shorter isoform. However, given the great homology between MICA and MICB, it is very likely that this antibody will detect both MICA and MICB. 3) ab61282 Based on the sequence of the peptide used to generate this antibody, it is 100% homologous to both isoforms of the human MICA protein and therefore should react to both on a western blot.  We have not done detailed experiments with this antibody to determine potential cross reactivity to  MICB (soluble or not), however the peptide used to generate this antibody is 86% homologous to MICB.  Depending on the amount of both proteins, this antibody will likely detect MICA over MICB, if it detects MICB at all. Although the answers may not be what you are expecting but I hope it could be of some use in determining if these products will be suitable for your research. If there is anything else that I could help you with please do not hesitate to contact me. Have a nice day! 

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