Key features and details
- Rabbit polyclonal to MICA
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MICA antibody
See all MICA primary antibodies
DescriptionRabbit polyclonal to MICA
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
A 16 amino acid peptide from near the carboxy terminus of human MICA.
- A549 cell lysate and A-20 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 43 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionSeems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Tissue specificityWidely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles. Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T cells.
Involvement in diseaseNote=Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma.
Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1) [MIM:177900]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
Sequence similaritiesBelongs to the MHC class I family. MIC subfamily.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
modificationsN-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.
Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.
Cellular localizationCell membrane. Cytoplasm. Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum.
- Information by UniProt
- HLA class I antigen antibody
- FLJ36918 antibody
- FLJ60820 antibody
Lane 1 : Anti-MICA antibody (ab62540) at 0.5 µg/ml
Lane 2 : Anti-MICA antibody (ab62540) at 1 µg/ml
Lane 3 : Anti-MICA antibody (ab62540) at 2 µg/ml
All lanes : A-20 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 43 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
ab62540 at 10µg/ml staining MICA in human colon tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human atheromatous aorta, staining MICA with ab62540.
Immunofluorescence of MICA in Human Colon cells using ab62540 at 20 ug/ml.
ab62540 has been referenced in 4 publications.
- Kerekes D et al. CD56+ immune cell infiltration and MICA are decreased in breast lobules with fibrocystic changes. Breast Cancer Res Treat 167:649-658 (2018). IHC-P ; Human . PubMed: 29090365
- Brandetti E et al. MYCN is an immunosuppressive oncogene dampening the expression of ligands for NK-cell-activating receptors in human high-risk neuroblastoma. Oncoimmunology 6:e1316439 (2017). PubMed: 28680748
- Moncayo G et al. MICA Expression Is Regulated by Cell Adhesion and Contact in a FAK/Src-Dependent Manner. Front Immunol 7:687 (2016). IHC . PubMed: 28154561
- Lin D et al. NF-?B Regulates MICA Gene Transcription in Endothelial Cell through a Genetically Inhibitable Control Site. J Biol Chem 287:4299-310 (2012). IHC-P ; Human . PubMed: 22170063