Recombinant Anti-MICA + MICB antibody [EPR6568] (ab150355)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6568] to MICA + MICB
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-MICA + MICB antibody [EPR6568]
See all MICA + MICB primary antibodies -
Description
Rabbit monoclonal [EPR6568] to MICA + MICB -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, A431, and MCF7 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6568 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 43-70 kDa (predicted molecular weight: 42 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 43-70 kDa (predicted molecular weight: 42 kDa). |
Target
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Function
Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. -
Tissue specificity
Widely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles. Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T-cells. -
Involvement in disease
Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma.
Psoriasis 1
Psoriatic arthritis -
Sequence similarities
Belongs to the MHC class I family. MIC subfamily.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.
Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation. -
Cellular localization
Cell membrane. Cytoplasm. Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 100507436 Human
- Entrez Gene: 4277 Human
- Omim: 600169 Human
- Omim: 602436 Human
- SwissProt: Q29980 Human
- SwissProt: Q29983 Human
- Unigene: 130838 Human
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Alternative names
- MHC class I chain-related protein A antibody
- MHC class I chain-related protein B antibody
- MHC class I polypeptide related sequence A antibody
see all
Images
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All lanes : Anti-MICA + MICB antibody [EPR6568] (ab150355) at 1/2000 dilution
Lane 1 : His-tagged human MICA recombinant protein, aa24-308
Lane 2 : His and Fc tagged human MICB recombinant protein, aa1-298
Lysates/proteins at 0.001 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 100,60 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
Exposure: 3 seconds (Lane 1), 10 seconds (Lane 2).
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Anti-MICA + MICB antibody [EPR6568] (ab150355) at 1/1000 dilution (purified) + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Blocking and diluting buffer : 5% NFDM/TBST -
Anti-MICA + MICB antibody [EPR6568] (ab150355) at 1/1000 dilution (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Blocking and diluting buffer : 5% NFDM/TBST -
All lanes : Anti-MICA + MICB antibody [EPR6568] (ab150355) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 43-70 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab150355 has been referenced in 3 publications.
- Seidel E et al. A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain. Nat Commun 12:2061 (2021). PubMed: 33824318
- Seidel E et al. Dynamic Co-evolution of Host and Pathogen: HCMV Downregulates the Prevalent Allele MICA*008 to Escape Elimination by NK Cells. Cell Rep N/A:N/A (2015). WB ; Human . PubMed: 25683719
- Campbell TM et al. Varicella-Zoster Virus and Herpes Simplex Virus 1 Differentially Modulate NKG2D Ligand Expression during Productive Infection. J Virol 89:7932-43 (2015). PubMed: 25995251