Overview

  • Product name

    Anti-MICAL1 antibody [EPR11486]
    See all MICAL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11486] to MICAL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MICAL1 aa 1000 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8TDZ2

  • Positive control

    • HeLa, A549, Human fetal lung and fetal kidney lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
IP 1/40 - 1/60.

Target

  • Function

    May be a cytoskeletal regulator that connects NEDD9 to intermediate filaments.
  • Tissue specificity

    Expressed in the thymus, lung, spleen, kidney, testis and hematopoietic cells.
  • Sequence similarities

    Contains 1 CH (calponin-homology) domain.
    Contains 1 LIM zinc-binding domain.
  • Domain

    The C-terminal coiled coil part contains the plexin-interacting region.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • CasL interacting molecule antibody
    • DKFZp434B1517 antibody
    • FLJ11937 antibody
    • FLJ21739 antibody
    • MICA1_HUMAN antibody
    • MICAL 1 antibody
    • MICAL antibody
    • MICAL1 antibody
    • microtubule associated monoxygenase, calponin and LIM domain containing 1 antibody
    • Molecule interacting with CasL protein 1 antibody
    • NEDD9 interacting protein with calponin homology and LIM domains antibody
    • NEDD9-interacting protein with calponin homology and LIM domains antibody
    • NICAL antibody
    • OTTHUMP00000016969 antibody
    see all

Images

  • All lanes : Anti-MICAL1 antibody [EPR11486] (ab181145) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 118 kDa

  • All lanes : Anti-MICAL1 antibody [EPR11486] (ab181145) at 1/10000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 118 kDa

  • Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181145 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

     

References

ab181145 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181145.
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