Product nameMicroglia Marker (CD11b, CD45, Iba1, TMEM119) Antibody Sampler Panel
Microglia Marker (CD11b, CD45, Iba1, TMEM119) Antibody Sampler Panel (ab226482) contains antibodies against the key microglia markers, CD11b, CD45, Iba1, and TMEM119.
Microglia are the macrophages of the brain and spinal cord and act as an immune defense in the central nervous system (CNS).
The rabbit monoclonal antibodies in this panel were selected for their exceptional performance in IHC, alongside other applications. Please see the individual datasheets for additional information.
Please note that Anti-TMEM119 antibody [28-3] is for mouse reactivity only.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
Storage instructionsPlease refer to protocols.
Components 1 packs ab133357 - Anti-CD11b antibody [EPR1344] 1 x 10µl ab40763 - Anti-CD45 antibody [EP322Y] 1 x 10µl ab178846 - Anti-Iba1 antibody [EPR16588] 1 x 10µl ab209064 - Anti-TMEM119 antibody [28-3] 1 x 10µl ab205718 - Goat Anti-Rabbit IgG H+L (HRP) 1 x 100µg
Cellular localizationCD11b: Membrane. CD45: Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. Iba1: Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. TMEM119: Membrane; Single-pass type I membrane protein
IHC image of TMEM119 staining in a section of frozen normal mouse brain. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in PBS containing 0.5% (v/v) Triton X-100, 0.3 M (w/v) glycine and 1% (w/v) BSA for 1 h at room temperature. The section was then incubated overnight at +4°C in PBS containing 0.5% (v/v) Triton X-100 and 1% (w/v) BSA with ab209064 at 0.5 µg/ml. The secondary antibody was ab150087 (shown in red) used at 2 µg/ml for 1 hour at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Immunohistochemical staining of paraffin embedded human tonsil with purified ab40763 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Formaldehyde-fixed, paraffin-embedded rat bone marrow tissue stained for CD11b using ab133357 at 1/5000 in immunohistochemical analysis.
Heat mediated antigen retrieval with EDTA buffer pH 9 was performed before commencing with staining protocol. 1% casein was used as blocking agent.
IHC image of Iba1 staining in mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab178846, 1/2000 dilution, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226482 has not yet been referenced specifically in any publications.