Product nameAnti-MID1 antibody
See all MID1 primary antibodies
DescriptionRabbit polyclonal to MID1
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide derived from an internal sequence of human MID1
- 293 cell extracts, human brain tissue and HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70770 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab70770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/500 - 1/1000.|
FunctionMay have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation.
Tissue specificityIn the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.
Involvement in diseaseDefects in MID1 are the cause of Opitz syndrome type I (OS-I) [MIM:300000]. OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. OS-I mutations produce proteins with a decreased affinity for microtubules.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain.
Contains 1 COS domain.
Contains 1 fibronectin type-III domain.
Contains 1 RING-type zinc finger.
modificationsPhosphorylated on serine and threonine residues.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.
- Information by UniProt
- BBBG 1 antibody
- BBBG1 antibody
- E3 ubiquitin-protein ligase Midline-1 antibody
All lanes : Anti-MID1 antibody (ab70770) at 1/500 dilution
Lane 1 : 293 extract
Lane 2 : 293 extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 75 kDa
Observed band size: 75 kDa
ab70770 at 1/500 dilution staining MID1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
ab70770 at 1/50 dilution staining MID1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
This product has been referenced in:
- Schweiger S et al. Resveratrol induces dephosphorylation of Tau by interfering with the MID1-PP2A complex. Sci Rep 7:13753 (2017). Read more (PubMed: 29062069) »
- Dierssen M et al. Reduced Mid1 Expression and Delayed Neuromotor Development in daDREAM Transgenic Mice. Front Mol Neurosci 5:58 (2012). WB ; Mouse . Read more (PubMed: 22563308) »