Overview

  • Product name

  • Description

    Rabbit polyclonal to MID1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human MID1

  • Positive control

    • 293 cell extracts, human brain tissue and HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab70770 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

Target

  • Function

    May have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation.
  • Tissue specificity

    In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.
  • Involvement in disease

    Defects in MID1 are the cause of Opitz syndrome type I (OS-I) [MIM:300000]. OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. OS-I mutations produce proteins with a decreased affinity for microtubules.
  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.
  • Information by UniProt
  • Database links

  • Alternative names

    • BBBG 1 antibody
    • BBBG1 antibody
    • E3 ubiquitin-protein ligase Midline-1 antibody
    • Finger on X and Y mouse homolog of antibody
    • FXY antibody
    • GBBB 1 antibody
    • GBBB1 antibody
    • MID 1 antibody
    • Mid1 antibody
    • Midin antibody
    • Midline 1 (Opitz/BBB syndrome) antibody
    • Midline 1 antibody
    • Midline 1 ring finger antibody
    • Midline 1 RING finger protein antibody
    • Midline-1 antibody
    • Midline1 antibody
    • OGS 1 antibody
    • OGS1 antibody
    • OS antibody
    • OSX antibody
    • Putative transcription factor XPRF antibody
    • RING finger protein 59 antibody
    • RING finger protein Midline-1 antibody
    • RNF 59 antibody
    • RNF59 antibody
    • TRI18 antibody
    • TRI18_HUMAN antibody
    • TRIM 18 antibody
    • TRIM18 antibody
    • Tripartite motif containing protein 18 antibody
    • Tripartite motif protein TRIM18 antibody
    • Tripartite motif-containing protein 18 antibody
    • XPRF antibody
    • zinc finger on X and Y, mouse, homolog of antibody
    • Zinc finger X and Y antibody
    • ZNFXY antibody
    see all

Images

  • All lanes : Anti-MID1 antibody (ab70770) at 1/500 dilution

    Lane 1 : 293 extract
    Lane 2 : 293 extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 75 kDa
    Observed band size: 75 kDa

  • ab70770 at 1/500 dilution staining MID1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab70770 at 1/50 dilution staining MID1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

References

This product has been referenced in:

  • Schweiger S  et al. Resveratrol induces dephosphorylation of Tau by interfering with the MID1-PP2A complex. Sci Rep 7:13753 (2017). Read more (PubMed: 29062069) »
  • Dierssen M  et al. Reduced Mid1 Expression and Delayed Neuromotor Development in daDREAM Transgenic Mice. Front Mol Neurosci 5:58 (2012). WB ; Mouse . Read more (PubMed: 22563308) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

DISCOUNT CODE: ************ Expiration date: DD MM YYYY Thank you for your interest in ab70770. The homology between the immunogen (aa 91-130 of human MID1 Uniprot ref O15344) and the Gallus gallus MID1 (Uniprot ref Q90WD1) is 87.5%. See attachment. It is likely that ab70770 can target the chick MID1. I am therefore very pleased to send you a testing discount code for you to test ab70770 in chicken. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for chicken and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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