Anti-MID2 antibody (ab14749)
Key features and details
- Goat polyclonal to MID2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MID2 antibody
See all MID2 primary antibodies -
Description
Goat polyclonal to MID2 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Monkey
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Immunogen
Synthetic peptide corresponding to Human MID2 aa 703-715.
Sequence:QESPYVSGMKTCH
(Peptide available asab23250) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab14749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 - 10 µg/ml. | |
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 81 kDa. A 1 hour primary incubation is recommended for this product. Approx 80-85kDa band observed in Mouse and Rat Heart, and Rat Kidney lysates (calculated MW of 77.8kDa according to Mouse NP_035975.1 and 83.1kDa according to Rat NP_001178818.1). |
Target
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Tissue specificity
Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain.
Contains 1 COS domain.
Contains 1 fibronectin type-III domain.
Contains 1 RING-type zinc finger. -
Domain
The tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization.
Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated. - Information by UniProt
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Database links
- Entrez Gene: 11043 Human
- Entrez Gene: 23947 Mouse
- Omim: 300204 Human
- SwissProt: Q9UJV3 Human
- SwissProt: Q9QUS6 Mouse
- Unigene: 12256 Human
- Unigene: 131097 Mouse
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Alternative names
- FLJ37715 antibody
- FLJ41813 antibody
- FXY2 antibody
see all
Images
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Western blot - Anti-MID2 antibody (ab14749)All lanes : Anti-MID2 antibody (ab14749) at 0.1 µg/ml
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat kidney lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 81 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID2 antibody (ab14749)ab14749 at 5ug/ml staining MID2 in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
Protocols
Datasheets and documents
References (0)
ab14749 has not yet been referenced specifically in any publications.
