Anti-MID2 antibody (ab14749)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-MID2 antibody
See all MID2 primary antibodies -
DescriptionGoat polyclonal to MID2
-
Host speciesGoat
-
Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
-
Species reactivityReacts with: Mouse, Human
Predicted to work with: Monkey
-
Immunogen
Synthetic peptide:
QESPYVSGMKTCH
, corresponding to amino acids 703-715 of Human MID2.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferPreservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab14749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 - 10 µg/ml. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 81 kDa.Can be blocked with Human MID2 peptide (ab23250). Approx 80-85kDa band observed in Mouse and Rat Heart, and Rat Kidney lysates (calculated MW of 77.8kDa according to Mouse NP_035975.1 and 83.1kDa according to Rat NP_001178818.1). |
Target
-
Tissue specificityLow level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
-
PathwayProtein modification; protein ubiquitination.
-
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain.
Contains 1 COS domain.
Contains 1 fibronectin type-III domain.
Contains 1 RING-type zinc finger. -
DomainThe tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization.
Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. -
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated.
- Information by UniProt
-
Database links
- Entrez Gene: 11043 Human
- Entrez Gene: 23947 Mouse
- Omim: 300204 Human
- SwissProt: Q9UJV3 Human
- SwissProt: Q9QUS6 Mouse
- Unigene: 12256 Human
- Unigene: 131097 Mouse
-
Alternative names
- FLJ37715 antibody
- FLJ41813 antibody
- FXY2 antibody
see all
Images
-
Western blot - Anti-MID2 antibody (ab14749)All lanes : Anti-MID2 antibody (ab14749) at 0.1 µg/ml
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat kidney lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 81 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID2 antibody (ab14749)ab14749 at 5ug/ml staining MID2 in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
Datasheets and documents
References
ab14749 has not yet been referenced specifically in any publications.
