Anti-MID2 antibody (ab14749)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MID2 antibody
See all MID2 primary antibodies -
Description
Goat polyclonal to MID2 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, ELISA, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Monkey
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Immunogen
Synthetic peptide corresponding to Human MID2 aa 703-715.
Sequence:QESPYVSGMKTCH
(Peptide available asab23250)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab14749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 - 10 µg/ml. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 81 kDa.Can be blocked with Human MID2 peptide (ab23250). Approx 80-85kDa band observed in Mouse and Rat Heart, and Rat Kidney lysates (calculated MW of 77.8kDa according to Mouse NP_035975.1 and 83.1kDa according to Rat NP_001178818.1). |
Target
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Tissue specificity
Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain.
Contains 1 COS domain.
Contains 1 fibronectin type-III domain.
Contains 1 RING-type zinc finger. -
Domain
The tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization.
Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated. - Information by UniProt
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Database links
- Entrez Gene: 11043 Human
- Entrez Gene: 23947 Mouse
- Omim: 300204 Human
- SwissProt: Q9UJV3 Human
- SwissProt: Q9QUS6 Mouse
- Unigene: 12256 Human
- Unigene: 131097 Mouse
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Alternative names
- FLJ37715 antibody
- FLJ41813 antibody
- FXY2 antibody
see all
Images
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Western blot - Anti-MID2 antibody (ab14749)All lanes : Anti-MID2 antibody (ab14749) at 0.1 µg/ml
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat kidney lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 81 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID2 antibody (ab14749)ab14749 at 5ug/ml staining MID2 in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
Datasheets and documents
References
ab14749 has not yet been referenced specifically in any publications.
