Overview

  • Product name

  • Description

    Rabbit Polyclonal to MID2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human MID2/KAI1 aa 506-735.
    Sequence:

    GTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQ MEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAI GIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLG VLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLP APDFIDYPERQECNCRPQESPYVSGMKTCH


    Database link: Q9UJV3-1

  • Positive control

    • IHC-P: Human kidney and pancreas tissue. ICC/IF: MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab236623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Tissue specificity

    Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
    Contains 1 RING-type zinc finger.
  • Domain

    The tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization.
    Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ37715 antibody
    • FLJ41813 antibody
    • FXY2 antibody
    • MID2 antibody
    • Midin 2 antibody
    • Midin-2 antibody
    • Midline 2 antibody
    • Midline defect 2 antibody
    • Midline-2 antibody
    • probable E3 ubiquitin protein ligase MID2 antibody
    • Probable E3 ubiquitin-protein ligase MID2 antibody
    • RING finger protein 60 antibody
    • Ring finger protein protein 60 antibody
    • RNF60 antibody
    • TRIM1 antibody
    • TRIM1_HUMAN antibody
    • tripartite motif containing protein 1 antibody
    • Tripartite motif protein 1 antibody
    • Tripartite motif-containing protein 1 antibody
    see all

Images

  • MCF7 (Human breast adenocarcinoma cell line) cells stained for MID2 (Green) using ab236623 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human pancreas tissue stained for MID2 with ab236623 at a 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human kidney tissue stained for MID2 with ab236623 at a 1/100 dilution in immunohistochemical analysis.

References

ab236623 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236623.
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