Key features and details
- Rabbit polyclonal to MID2 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MID2 antibody - C-terminal
See all MID2 primary antibodies
DescriptionRabbit polyclonal to MID2 - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human MID2 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q9UJV3
- WB: MID2 transfected HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 83 kDa.|
Tissue specificityLow level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain.
Contains 1 COS domain.
Contains 1 fibronectin type-III domain.
Contains 1 RING-type zinc finger.
DomainThe tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization.
Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain.
modificationsPhosphorylated on serine and threonine residues.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated.
- Information by UniProt
- FLJ37715 antibody
- FLJ41813 antibody
- FXY2 antibody
All lanes : Anti-MID2 antibody - C-terminal (ab228612) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T(human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : MID2 transfected HEK-293T whole cell lysate
Predicted band size: 83 kDa
12% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228612 has not yet been referenced specifically in any publications.