Overview

  • Product name

    Anti-MID2 antibody - C-terminal
    See all MID2 primary antibodies
  • Description

    Rabbit polyclonal to MID2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human MID2 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: Q9UJV3

  • Positive control

    • WB: MID2 transfected HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.

Target

  • Tissue specificity

    Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
    Contains 1 RING-type zinc finger.
  • Domain

    The tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization.
    Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ37715 antibody
    • FLJ41813 antibody
    • FXY2 antibody
    • MID2 antibody
    • Midin 2 antibody
    • Midin-2 antibody
    • Midline 2 antibody
    • Midline defect 2 antibody
    • Midline-2 antibody
    • probable E3 ubiquitin protein ligase MID2 antibody
    • Probable E3 ubiquitin-protein ligase MID2 antibody
    • RING finger protein 60 antibody
    • Ring finger protein protein 60 antibody
    • RNF60 antibody
    • TRIM1 antibody
    • TRIM1_HUMAN antibody
    • tripartite motif containing protein 1 antibody
    • Tripartite motif protein 1 antibody
    • Tripartite motif-containing protein 1 antibody
    see all

Images

  • All lanes : Anti-MID2 antibody - C-terminal (ab228612) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T(human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : MID2 transfected HEK-293T whole cell lysate

    Predicted band size: 83 kDa



    12% SDS-PAGE gel.

References

ab228612 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228612.
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