Product nameAnti-Midkine antibody
See all Midkine primary antibodies
DescriptionRabbit polyclonal to Midkine
Tested applicationsSuitable for: IHC-FoFr, WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Cow
- Purchase matching WB positive control:Recombinant Human Midkine protein
- ab36038 gave a positive result against a fusion protein consisting of full-length Midkine protein with a tag and in DU145 cell line.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab36038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 16 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionHas heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.
Sequence similaritiesBelongs to the pleiotrophin family.
- Information by UniProt
- Amphiregulin associated protein antibody
- Amphiregulin-associated protein antibody
- ARAP antibody
Anti-Midkine antibody (ab36038) at 1 µg/ml + Recombinant Midkine protein with tag at 0.1 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
The observed molecular weight of 42 kDa is the combined molecular weight of the full-length recombinant Midkine protein (~140 amino-acids) and the tag.
IHC-FoFr image of Midkine (ab36038) staining on Rat Spinal cord sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the cords were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat and the immunostainings were preformed using the ‘free floating’ technique. Image B is a high power magnification of Image A.
ICC/IF image of ab36038 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36038, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- He D et al. Ethanol activates midkine and anaplastic lymphoma kinase signaling in neuroblastoma cells and in the brain. J Neurochem 135:508-21 (2015). Read more (PubMed: 26206265) »
- Florea V et al. c-Myc Is Essential to Prevent Endothelial Pro-Inflammatory Senescent Phenotype. PLoS One 8:e73146 (2013). Read more (PubMed: 24039874) »