Overview

  • Product name

    Anti-Midkine antibody - C-terminal
    See all Midkine primary antibodies
  • Description

    Rabbit polyclonal to Midkine - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Midkine aa 110-143 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P21741

  • Positive control

    • Mouse kidney tissue lysate; Human pancreas tissue; HeLa and HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Purification notes

    ab170820 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 16 kDa.
ICC/IF 1/10 - 1/50.

Target

  • Function

    Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.
  • Sequence similarities

    Belongs to the pleiotrophin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Amphiregulin associated protein antibody
    • Amphiregulin-associated protein antibody
    • ARAP antibody
    • FLJ27379 antibody
    • Mdk antibody
    • Midgestation and kidney protein antibody
    • Midkine antibody
    • MK 1 antibody
    • MK antibody
    • MK_HUMAN antibody
    • MK1 antibody
    • NEGF 2 antibody
    • NEGF2 antibody
    • Neurite growth promoting factor 2 antibody
    • Neurite outgrowth promoting protein antibody
    • Neurite outgrowth-promoting factor 2 antibody
    • Neurite outgrowth-promoting protein antibody
    • Retinoic acid inducible factor antibody
    see all

Images

  • Anti-Midkine antibody - C-terminal (ab170820) at 1/100 dilution + Mouse kidney tissue lysate at 35 µg

    Predicted band size: 16 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Midkine with ab170820 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Confocal immunofluorescent analysis of HeLa cells labeling Midkine with ab170820 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Confocal immunofluorescent analysis of HepG2 cells labeling Midkine with ab170820 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

References

This product has been referenced in:

  • Meng X  et al. DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma. EBioMedicine 41:185-199 (2019). Read more (PubMed: 30773478) »
See 1 Publication for this product

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