Key features and details
- Rabbit polyclonal to MIDN
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MIDN antibody
See all MIDN primary antibodies
DescriptionRabbit polyclonal to MIDN
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- IHC-P: Human kidney tissue; WB: Human tonsil tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Our Abpromise guarantee covers the use of ab184825 in the following tested applications.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 49 kDa.|
FunctionMay be involved in regulation of genes related to neurogenesis in the nucleolus.
Sequence similaritiesContains 1 ubiquitin-like domain.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- Midbrain nucleolar protein antibody
- midn antibody
- MIDN_HUMAN antibody
- Midnolin antibody
Immunohistochemical analysis of human kidney tssue labeling MIDN in the nucleus of cells in tubules with ab184825 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-MIDN antibody (ab184825) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma (IgG/HSA depleted) lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 49 kDa
ab184825 has not yet been referenced specifically in any publications.