Key features and details
- Rabbit polyclonal to MIER3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MIER3 antibody
See all MIER3 primary antibodies
DescriptionRabbit polyclonal to MIER3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment, corresponding to a region within amino acids 234-476 of Human MIER3.
- 293T, A431, Jurkat and Raji whole cell lysates; Rat RT2, Human Huh7 and Mouse C2C12 xenograft tissues.
General notesKeep as concentrated solution.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab127688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/100 - 1/500.|
Sequence similaritiesContains 1 ELM2 domain.
Contains 1 SANT domain.
- Information by UniProt
- DKFZp686L09111 antibody
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Anti-MIER3 antibody (ab127688) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 61 kDa
7.5% SDS PAGE
ab127688, at a dilution of 1/500, staining MIER3 in paraffin-embedded Rat RT2 xenograft tissue by Immunohistochemistry.
ab127688, at a dilution of 1/500, staining MIER3 in paraffin-embedded Human Huh7 xenograft tissue by Immunohistochemistry.
ab127688, at a dilution of 1/500, staining MIER3 in paraffin-embedded Mouse C2C12 xenograft tissue by Immunohistochemistry.
ab127688 has been referenced in 1 publication.
- Peng M et al. MIER3 suppresses colorectal cancer progression by down-regulating Sp1, inhibiting epithelial-mesenchymal transition. Sci Rep 7:11000 (2017). PubMed: 28887525