Key features and details
- Chicken polyclonal to MIF
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgY
Product nameAnti-MIF antibody
See all MIF primary antibodies
DescriptionChicken polyclonal to MIF
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Pig, Cynomolgus monkey, Macaque monkey
- Supernatants or lysates of endotoxin-stimulated human peripheral blood mononuclear cells (PBMC).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesThis product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
Our Abpromise guarantee covers the use of ab34644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/1000 - 1/2000.|
|WB||1/500 - 1/1000. Detects a band of approximately 12.3 kDa (predicted molecular weight: 14 kDa).|
FunctionPro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Involvement in diseaseGenetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Sequence similaritiesBelongs to the MIF family.
Cellular localizationSecreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
- Information by UniProt
- GIF antibody
- GLIF antibody
- Glycosylation inhibiting factor antibody
All lanes : Anti-MIF antibody (ab34644) at 1/500 dilution
Lane 1 : Control
Lane 2 : 100 ng of recombinant
MIF present in a bacterial lysate
Lane 2 : Goat anti chicken at 1/5000 dilution
Predicted band size: 14 kDa
Observed band size: 12.3 kDa why is the actual band size different from the predicted?
ab34644 has not yet been referenced specifically in any publications.