Anti-MIF antibody (ab7207)
Key features and details
- Rabbit polyclonal to MIF
- Suitable for: WB, IHC-Fr, ICC/IF, ELISA, IP, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-MIF antibody
See all MIF primary antibodies -
Description
Rabbit polyclonal to MIF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, ICC/IF, ELISA, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Rat MIF.
Database link: P30904 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
|
IHC-Fr | (3) |
Use at an assay dependent concentration.
|
ICC/IF | (1) |
Use at an assay dependent concentration.
|
ELISA | (1) |
Use at an assay dependent concentration. PubMed: 19066630
|
IP |
Use at an assay dependent concentration.
|
|
IHC-P | (2) |
Use at an assay dependent concentration.
|
Notes |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. PubMed: 19066630 |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Target
-
Function
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. -
Involvement in disease
Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. -
Sequence similarities
Belongs to the MIF family. -
Cellular localization
Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. - Information by UniProt
-
Database links
- Entrez Gene: 17319 Mouse
- Entrez Gene: 81683 Rat
- SwissProt: P34884 Mouse
- SwissProt: P30904 Rat
- Unigene: 2326 Mouse
- Unigene: 2661 Rat
-
Alternative names
- GIF antibody
- GLIF antibody
- Glycosylation inhibiting factor antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (37)
ab7207 has been referenced in 37 publications.
- Santos LC et al. Kisspeptin/Kiss1r system and angiogenic and immunological mediators at the maternal-fetal interface of domestic cats. Biol Reprod 105:217-231 (2021). PubMed: 33774655
- Gosuen Gonçalves Dias F et al. Topical formulations containing Copaifera duckei Dwyer oleoresin improve cutaneous wound healing. Avicenna J Phytomed 11:120-133 (2021). PubMed: 33907671
- Jin C et al. A Unique Type of Highly-Activated Microglia Evoking Brain Inflammation via Mif/Cd74 Signaling Axis in Aged Mice. Aging Dis 12:2125-2139 (2021). PubMed: 34881090
- Chen W et al. Exosomal MIF Derived From Nasopharyngeal Carcinoma Promotes Metastasis by Repressing Ferroptosis of Macrophages. Front Cell Dev Biol 9:791187 (2021). PubMed: 35036405
- Kreutzberger AJB et al. Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2. Elife 9:N/A (2020). PubMed: 33164744