Overview

  • Product name

  • Description

    Rabbit polyclonal to MIF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, ELISA, IHC-Fr, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein (Rat)(E. coli-expressed).

  • Positive control

    • LPS stimulated RAW cell lysate Neuro2A (mouse neuroblastoma cell line) lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
ELISA Use at an assay dependent concentration. PubMed: 19066630
IHC-Fr Use at an assay dependent concentration.
WB 1/2000.
IP 1/500.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • Involvement in disease

    Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
  • Sequence similarities

    Belongs to the MIF family.
  • Cellular localization

    Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Information by UniProt
  • Database links

  • Alternative names

    • GIF antibody
    • GLIF antibody
    • Glycosylation inhibiting factor antibody
    • Glycosylation-inhibiting factor antibody
    • L-dopachrome isomerase antibody
    • L-dopachrome tautomerase antibody
    • Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
    • Macrophage migration inhibitory factor antibody
    • MIF antibody
    • MIF protein antibody
    • MIF_HUMAN antibody
    • MMIF antibody
    • Phenylpyruvate tautomerase antibody
    see all

Images

  • Anti-MIF antibody (ab7207) + LPS stimulated RAW cell lysate

    Performed under reducing conditions.


    MIF detected by Western Blot in LPS stimulated RAW cell lysate.

  • ab7207 staining MIF in Mouse small intestine tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with Dako protein block for 1 hour at room temperature. Samples were incubated with primary antibody (1/200) for 1 hour. An undiluted Alkaline Phosphatase-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • ab7207 staining MIF in Rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% protein blocking solution for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate EDTA buffer (pH 6.2). Samples were incubated with primary antibody (1/200 in antibody diluent) for 1 hour. An undiluted Alkaline phosphatase-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • ab7207 staining MIF in murine immortalised bone marrow-derived macrophages by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/250 in 5% goat serum/3% BSA in PBS) for 1 hour at 20°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.
  • All lanes : Anti-MIF antibody (ab7207) at 1/1000 dilution

    Lanes 1-2 : Whole cell lysate prepared from normal rat serum
    Lanes 3-4 : Whole cell lysate prepared from rat serum (90 minutes warm ischemia and 1h reperfusion)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Developed using the ECL technique.

    Observed band size: 13 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes

    See Abreview

  • From a review by Arsi Rosengren.
  • ab7207 staining mouse Testis tissue sections by Immunohistochemistry (Frozen sections). Tissue was fixed in paraformaldehyde and blocked in 1% serum for 10 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with sample for 20 hours at 20°C. A biotin labelled goat polyclonal to rabbit IgG antibody, diluted 1/500 was used as the secondary.

    See Abreview

  • ab7207 used at a 1/1000 dilution as detection antibody in ELISA testing MIF in rat serum samples. Samples were blocked with 1% BSA for 2 hours at room temperature. The secondary used was an HRP conjugated donkey anti-goat (H+L) polyclonal used at a 1/200,000 dilution.

    See Abreview

References

This product has been referenced in:

  • Ha W  et al. Ibudilast sensitizes glioblastoma to temozolomide by targeting Macrophage Migration Inhibitory Factor (MIF). Sci Rep 9:2905 (2019). Read more (PubMed: 30814573) »
  • Shveiky D  et al. Age-associated impairments in tissue strength and immune response in a rat vaginal injury model. Int Urogynecol J N/A:N/A (2019). Read more (PubMed: 31243497) »
See all 26 Publications for this product

Customer reviews and Q&As

1-4 of 4 Q&A

Answer

Thank you for confirming the nature of the problem and also the previous order details, I was able to find that the new vial is from a different batch, though we have not received other complaints about this antibody at all so it is likely that the antibody was damaged during shipping or storage. According to the order details for the problematic vial there was two vials ordered on order number 86047 (PO 767795), could you please tell me if both vials gave the same problem or just one of them? My apologies for the delay in arranging the replacement(s), thank you in advance for your cooperation,

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Question
Answer

This antibody has not been tested for imunocytochemistry in rat tissue, therefore we can't guarantee results.

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Question
Answer

This antibody has not been tested in species other than rat and mouse. However, depending on the homology between species it may work in human tissue.

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Question
Answer

This antibody was raised against full-length recombinant MIF, so it has a good chance of being a blocking Ab. However, it has not yet been tested.

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