Western blot abreview for Anti-MIF antibody

Good
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Serum)
Loading amount
10 µg
Specification
Serum
Treatment
90 minutes warm ischemia and 1h reperfusion
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5% BSA in PBST

Secondary antibody

Dilution
1/5000

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: about 13 kDa

Additional data

Additional Notes
Serum samples were diluted to 1/5 in PBS. the loading amount was 10ul.

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Submitted Jun 20 2011

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