Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12462] to MIF
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Product nameAnti-MIF antibody [EPR12462]
See all MIF primary antibodies
DescriptionRabbit monoclonal [EPR12462] to MIF
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Chicken, Cow, Cynomolgus monkey, Rhesus monkey
Synthetic peptide within Human MIF aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P14174
- Y79, Jurkat, A375 and THP1 cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab176565 in the following tested applications.
|WB||1/1000 - 1/10000. Predicted molecular weight: 12 kDa.|
|IP||1/10 - 1/100.|
FunctionPro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Involvement in diseaseGenetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Sequence similaritiesBelongs to the MIF family.
Cellular localizationSecreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
- Information by UniProt
- GIF antibody
- GLIF antibody
- Glycosylation inhibiting factor antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: MIF knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab176565 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab176565 was shown to specifically recognize MIF in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when knockout cells were examined. Wild-type and MIF knockout samples were subjected to SDS-PAGE. Ab176565 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-MIF antibody [EPR12462] (ab176565) at 1/1000 dilution
Lane 1 : Y79 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A375 cell lysate
Lane 4 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate labeling MIF with ab176565 at 1/10 dilution.
ab176565 has been referenced in 1 publication.
- Takiya CS et al. Proteomic analysis reveals greater abundance of complement and inflammatory proteins in subcutaneous adipose tissue from postpartum cows treated with sodium salicylate. J Proteomics 204:103399 (2019). PubMed: 31152939