Key features and details
- Rabbit polyclonal to MIG-6
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MIG-6 antibody
See all MIG-6 primary antibodies
DescriptionRabbit polyclonal to MIG-6
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human MIG-6 (internal sequence). NP_061821.
Database link: Q9UJM3
- Human liver cancer tissue; Human brain malignant glioma tissue lysate; HepG2 whole cell lysate (ab7900)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab198834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/100.|
|WB||1/200 - 1/1000. Predicted molecular weight: 51 kDa.|
FunctionNegative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development.
Sequence similaritiesBelongs to the MIG6 family.
DomainThe EGFR-binding region prevents binding of a cyclin-like activator to the EGFR kinase domain, and thereby keeps EGFR in an inactive conformation. Also maintains EGFR in an inactive conformation by preventing formation of an asymmetric homodimer.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Associated with the plasma membrane of basal skin keratinocytes. Translocates into the nucleus of differentiating suprabasal keratinocytes.
- Information by UniProt
- ERBB receptor feedback inhibitor 1 antibody
- ERRFI_HUMAN antibody
- Errfi1 antibody
All lanes : Anti-MIG-6 antibody (ab198834) at 1/400 dilution
Lane 1 : Human brain malignant glioma tissue lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 51 kDa
Exposure time: 5 minutes
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling MIG-6 with ab198834 at 1/35 dilution.
ab198834 has been referenced in 1 publication.
- Cairns J et al. Differential roles of ERRFI1 in EGFR and AKT pathway regulation affect cancer proliferation. EMBO Rep 19:N/A (2018). PubMed: 29335246