Key features and details
- Rabbit polyclonal to MIG-6
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MIG-6 antibody
See all MIG-6 primary antibodies
DescriptionRabbit polyclonal to MIG-6
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human MIG-6 (internal sequence). The exact sequence is proprietary.
Database link: Q9UJM3
- WB: HCT 116 whole cell lysate. IHC-P: Mouse placenta tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 51 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionNegative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development.
Sequence similaritiesBelongs to the MIG6 family.
DomainThe EGFR-binding region prevents binding of a cyclin-like activator to the EGFR kinase domain, and thereby keeps EGFR in an inactive conformation. Also maintains EGFR in an inactive conformation by preventing formation of an asymmetric homodimer.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Associated with the plasma membrane of basal skin keratinocytes. Translocates into the nucleus of differentiating suprabasal keratinocytes.
- Information by UniProt
- ERBB receptor feedback inhibitor 1 antibody
- ERRFI_HUMAN antibody
- Errfi1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227944 has been referenced in 2 publications.
- Pan R et al. Inhibition of KHSRP sensitizes colorectal cancer to 5-fluoruracil through miR-501-5p-mediated ERRFI1 mRNA degradation. J Cell Physiol N/A:N/A (2019). PubMed: 31313286
- Liang R et al. High expression of estrogen-related receptor a is significantly associated with poor prognosis in patients with colorectal cancer. Oncol Lett 15:5933-5939 (2018). PubMed: 29552224