Overview

  • Product name

  • Description

    Rabbit polyclonal to MIG-6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human MIG-6 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UJM3

  • Positive control

    • WB: HCT 116 whole cell lysate. IHC-P: Mouse placenta tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Negative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development.
  • Sequence similarities

    Belongs to the MIG6 family.
  • Domain

    The EGFR-binding region prevents binding of a cyclin-like activator to the EGFR kinase domain, and thereby keeps EGFR in an inactive conformation. Also maintains EGFR in an inactive conformation by preventing formation of an asymmetric homodimer.
  • Cellular localization

    Cytoplasm. Cell membrane. Nucleus. Associated with the plasma membrane of basal skin keratinocytes. Translocates into the nucleus of differentiating suprabasal keratinocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • ERBB receptor feedback inhibitor 1 antibody
    • ERRFI_HUMAN antibody
    • Errfi1 antibody
    • GENE 33 antibody
    • Gene 33, rat, homolog of antibody
    • Mig-6 antibody
    • MIG6 antibody
    • mitogen inducible gene 6 antibody
    • mitogen inducible gene 6 protein antibody
    • Mitogen-inducible gene 6 protein antibody
    • RALT antibody
    • receptor-associated late transducer antibody
    see all

Images

  • Anti-MIG-6 antibody (ab227944) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 51 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded mouse placenta tissue stained for MIG-6 using ab227944 at 1/500 dilution in immunohistochemical analysis.

References

This product has been referenced in:

  • Liang R  et al. High expression of estrogen-related receptor a is significantly associated with poor prognosis in patients with colorectal cancer. Oncol Lett 15:5933-5939 (2018). Read more (PubMed: 29552224) »
See 1 Publication for this product

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