Overview

  • Product name
  • Description
    Rabbit polyclonal to MIG
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, Neutralising, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hMIG (human Monokine induced by Interferon-Gamma)

  • Positive control
    • Recombinant human MIG protein (ab9721) can be used as a positive control in WB.

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.00 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required
WB Use 0.1µg for 100.2 cells.
IHC-P Use a concentration of 0.25 - 0.5 µg/ml.

Target

  • Function
    Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C motif chemokine 9 antibody
    • chemokine (C-X-C motif) ligand 9 antibody
    • CMK antibody
    • crg-10 antibody
    • CXCL9 antibody
    • CXCL9_HUMAN antibody
    • gamma interferon induced monokine antibody
    • Gamma-interferon-induced monokine antibody
    • HuMIG antibody
    • MIG antibody
    • monokine induced by gamma interferon antibody
    • monokine induced by interferon gamma antibody
    • Monokine induced by interferon-gamma antibody
    • SCYB9 antibody
    • Small inducible cytokine B9 antibody
    • small inducible cytokine subfamily B member 9 antibody
    • Small-inducible cytokine B9 antibody
    see all

Images

  • ab9720 staining MIG in human renal tumor with parenchyma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • ab9720 staining MIG in Human tonsil (periphery) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 5 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) for 30 minutes at 25°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Liu W  et al. Elevated expression of IFN-inducible CXCR3 ligands predicts poor prognosis in patients with non-metastatic clear-cell renal cell carcinoma. Oncotarget 7:13976-83 (2016). IHC . Read more (PubMed: 26910919) »
  • Chmielewski S  et al. STAT1-dependent signal integration between IFN? and TLR4 in vascular cells reflect pro-atherogenic responses in human atherosclerosis. PLoS One 9:e113318 (2014). IHC-P ; Human . Read more (PubMed: 25478796) »
See all 3 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting us. The product is shipped as 100 ug of lyophilized antibody. If reconstituted in 200 ul of water, the concentration will be 0.5 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us. We do not currently have any feedback from customers or lab testing regarding whether it would be possible to conjugate either of these antibodies to gold, and we therefore do not have any recommendations for a pH or concentration for the conjuagtion process. I am sorry I could not be more helpful, please let me know if you need any additional information or assistance.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil (periphery))
Specification
Tonsil (periphery)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
(agent) for 5 minute(s) · Concentration: 100% · Temperature: 25°C

Dr. R Berahovich

Verified customer

Submitted Dec 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colorectal carcinoma)
Specification
Colorectal carcinoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 10 2011

Answer

Thank you for contacting us for technical support regarding ab9720. This antibody has not been tested on mouse tissue therefore we do not know what sort of banding pattern it gives with MIG of this species I'm afraid. There is 73% homology between the human protein and the mouse protein, so the antibody may react to the mouse protein. The problem of non specific bands could be due to a number of reasons: -degradation of the protein in your mouse brain lysates if there is not enough protease inhibitors in the lysis buffer -blocking conditions are not adequate (too much or not not enough blocking, wrong blocking buffer) -the antibody could maybe be diluted more? I would be happy to look at your protocol in detail to provide more specific recommendations. To help you put your protocol together easily we have a short questionnaire accessible via this link: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=9720&mode=questionaire If you can please put it to my attention I will personally look into this matter. I would encourage you to submit an Abreview to share your findings with other researchers, via the online datasheet (tab Abreview). The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for rewards such as Amazon.com gift certificates or discounts on future antibody purchases.

Read More

Question
Answer

Unfortunately, this antibody has not been tested for IHC in frozen sections, so we can't guarantee that it will work.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up