Overview

  • Product name
  • Description
    Rabbit polyclonal to Migfilin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment corresponding to Human Migfilin aa 171-373.

  • Positive control
    • HCT116 whole cell lysate; Human MDAMB231 xenograft tissue.
  • General notes

    Previously labelled as FBLIM1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab154417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function
    Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin-containing signaling complexes that control actin assembly.
  • Tissue specificity
    Isoform 1 and isoform 3 are expressed in heart, kidney, lung, pancreas, placenta and platelets. Isoform 2 is expressed in brain, heart, kidney, lung, pancreas, placenta, skeletal muscle and platelets.
  • Sequence similarities
    Contains 3 LIM zinc-binding domains.
  • Cellular localization
    Cytoplasm > cell cortex. Cell junction > focal adhesion. Cytoplasm > cytoskeleton. Associated with actin stress fiber at cell-ECM focal adhesion sites. Isoform 1 and isoform 3 are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. Isoform 2 is localized at actin stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410043F08Rik antibody
    • Cal antibody
    • CSX associated LIM antibody
    • DKFZp434G171 antibody
    • FBLI1_HUMAN antibody
    • FBLIM 1 antibody
    • Fblim1 antibody
    • FBLP 1 antibody
    • FBLP-1 antibody
    • FBLP1 antibody
    • Filamin binding LIM protein 1 antibody
    • Filamin-binding LIM protein 1 antibody
    • Gt10 antibody
    • MGC105665 antibody
    • MIG2 interacting protein; antibody
    • MIG2-interacting protein antibody
    • Migfilin antibody
    • Mitogen inducible 2 interacting protein antibody
    • Mitogen-inducible 2-interacting protein antibody
    • OTTHUMP00000003118 antibody
    • OTTHUMP00000003119 antibody
    • OTTHUMP00000003120 antibody
    • OTTMUSP00000011062 antibody
    • OTTMUSP00000011063 antibody
    • RP11-169K16.5 antibody
    • RP23-291H3.1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded MDAMB231 xenograft labeling Migfilin with ab154417 at 1/250 dilution.

  • Anti-Migfilin antibody (ab154417) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 41 kDa



    10% SDS PAGE

References

ab154417 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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