Anti-MIIP antibody (ab167197)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MIIP antibody
See all MIIP primary antibodies -
Description
Mouse polyclonal to MIIP -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein, corresponding to amino acids 1-388 of Human MIIP (AAH08068.1).
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Positive control
- Lysate of 293T cells transfected with MIIP.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab167197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. |
Target
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Relevance
MIIP has 3 SEG (segments of low compositional complexity) domains, an RGD motif, and several potential phosphorylation sites. The C-terminal region of IGFBP2 interacts with a central 44-amino acid sequence of MIIP. MIIP inhibits glioma cells invasion and down-regulates adhesion- and motility-associated genes such as NFKB2 and ICAM1. It exhibits opposing effects to IGFBP2 on cell invasion. There are 2 named isoforms due to alternative splicing. Isoform 1 is expressed in brain but underexpressed in glioma tissues, at protein level. Isoform 2 is not detected in normal organs, but is expressed in gliomas with increasing levels with glioma progression. On the contrary, at protein level, isoform 2 is not detected in gliomas, suggesting that this isoform is unstable in glioma cells. Isoform 2 is degraded by the ubiquitin-proteasome pathway. -
Database links
- Entrez Gene: 60672 Human
- Omim: 608772 Human
- SwissProt: Q5JXC2 Human
- Unigene: 8595 Human
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Alternative names
- FLJ12438 antibody
- FLJ38609 antibody
- IGFBP 2 binding protein antibody
see all
Images
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Western blot - Anti-MIIP antibody (ab167197)All lanes : Anti-MIIP antibody (ab167197) at 1 µg/ml
Lane 1 : Lysate of 293T cells transfected with MIIP
Lane 2 : Lysate of 293T cells (non-transfected)
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-mouse HRP conjugated antibody at 1/2500 dilution
Predicted band size: 43 kDa
Protocols
Datasheets and documents
References
ab167197 has not yet been referenced specifically in any publications.
