Overview

  • Product name
    Anti-Milk Fat Globule 1 antibody [SPM291]
    See all Milk Fat Globule 1 primary antibodies
  • Description
    Mouse monoclonal [SPM291] to Milk Fat Globule 1
  • Host species
    Mouse
  • Specificity
    We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • SKBR-3 cells. Breast, colon, or lung tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab17787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40-45 kDa (predicted molecular weight: 40-45 kDa).
IP Use at 2 µg/mg of lysate.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
    Medin is the main constituent of aortic medial amyloid.
  • Tissue specificity
    Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
  • Sequence similarities
    Contains 1 EGF-like domain.
    Contains 2 F5/8 type C domains.
  • Post-translational
    modifications
    Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
  • Cellular localization
    Membrane. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BA46 antibody
    • Breast epithelial antigen BA46 antibody
    • EDIL1 antibody
    • HMFG antibody
    • hP47 antibody
    • HsT19888 antibody
    • Lactadherin antibody
    • Medin antibody
    • MFG-E8 antibody
    • MFGE8 antibody
    • MFGM antibody
    • MFGM_HUMAN antibody
    • Milk fat globule EGF factor 8 antibody
    • Milk fat globule EGF factor 8 protein antibody
    • Milk fat globule-EGF factor 8 antibody
    • O acetyl disialoganglioside synthase antibody
    • OAcGD3S antibody
    • SED1 antibody
    • SPAG10 antibody
    • Sperm associated antigen 10 antibody
    • Sperm surface protein hP47 antibody
    see all

Images

  • ab17787 Milk Fat Globule Membrane Protein staining in human tissue. The primary antibody was diluted 1/50, and incubated for 30 minutes at room temperature.
  • IHC image of ab17787 staining in human normal breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17787, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing MCF-7 cells stained with ab17787 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17787, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Yu L  et al. MFGE8 regulates TGF-ß-induced epithelial mesenchymal transition in endometrial epithelial cells in vitro. Reproduction 152:225-33 (2016). ICC/IF, WB ; Human . Read more (PubMed: 27340235) »
  • Soki FN  et al. Polarization of Prostate Cancer-associated Macrophages Is Induced by Milk Fat Globule-EGF Factor 8 (MFG-E8)-mediated Efferocytosis. J Biol Chem 289:24560-72 (2014). IHC-P ; Human . Read more (PubMed: 25006249) »
See all 4 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (MCF7 cells)
Specification
MCF7 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 07 2015

Answer


I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.
Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.
The credit note ID is for your reference only and does not automatically guarantee the credit.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thanks for your email. ab4587 does not have any required MSDS information, so a link will not be present on this datasheet. For example, ab7254 does have a link to MSDS information. I hope this helps. Please contact me again if you have any further questions. 

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Answer

Thanks for your enquiry. If required, MSDS information is located via one or more 'MSDS' links near the top left corner of the online datasheet. This link is just below the 'Datasheet PDF' link present on all datasheets. I hope this is helpful. Please contact me again if you have any further questions.

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Answer

Nos anticorps anti hMFG1 sont des anticorps monoclonaux contre la protéine totale hMFG1, et nous ne savons pas ou se situe l'épitope. Cependant, j'ai effectué une recherche d'homologie entre les formes 1 et 8 et vous trouverez en attachement les résultats de celle-ci. La forme hMFG1 est composée de 335 acides aminés, la forme hMFG8 de ces même 335 acides aminés, plus une partie non homologue, ce qui forme au total une protéine de 387 acides aminés. Comme les anticorps monoclonaux sont en fait contre une partie identique aux deux protéines, nous pouvons prédire qu'ils réagiront également contre hMFG8. Comme cela n'a pas été testé je ne peux pas vous le garantir, mais j'espère que ces données vous aiderons dans votre recherche.

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Answer

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. We do, however, have our Abreview system, where researchers can submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for rewards such as Amazon.com gift certificates or discounts on future antibody purchases. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. In addition, our Abpromise states that if the antibody does not perform as described on this datasheet, notify us within 90 days of delivery, so that we can help you or offer you a replacement or a refund. The datasheet states that the antibody will recognize lactadherin by Western blot, so if you do have a problem please contact us. If we cannot troubleshoot your problem, we will offer you a replacement, credit note, or refund. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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