Product nameMilk Fat Globule 1 overexpression 293T lysate (whole cell)
ab94238 is a 293T cell transfected lysate in which Human Human Milk Fat Globule 1 has been transiently over-expressed using a pCMV-Human Milk Fat Globule 1 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. Medin is the main constituent of aortic medial amyloid. Tissue specificity: Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas. Similarity: Contains 1 EGF-like domain. Contains 2 F5/8 type C domains. PTM: Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
Our Abpromise guarantee covers the use of ab94238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94238 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Milk Fat Globule 1 antibody (ab89232) at 1/500 dilution
Lane 1 :
Milk Fat Globule 1 overexpression 293T lysate (whole cell) (ab94238)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94238 has not yet been referenced specifically in any publications.