Product nameMilnacipran hydrochloride, Serotonin and norepinephrine reuptake inhibitor
DescriptionSerotonin and norepinephrine reuptake inhibitor
Biological descriptionSerotonin and norepinephrine reuptake inhibitor. IC50 values are 100, 203 and >100000 nM for NA, 5-HT and DA, respectively. Centrally active following oral administration. Exhibits antidepressant and antifibromyalgia activity in vivo.
Chemical name(1R*,2S*)-2-(Aminomethyl)-N,N-diethyl-1-phenylcyclopropanecarboxamide hydrochloride
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 100 mM and in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
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Our Abpromise guarantee covers the use of ab120755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113202 staining NET1 in PC-12 cells treated with milnacipran hydrochloride (ab120755), by ICC/IF. Decrease of NET1 expression correlates with increased concentration of milnacipran hydrochloride, as described in literature.
The NGF treated cells were incubated at 37°C for 6 hour in media containing different concentrations of ab120755 (milnacipran hydrochloride ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab113202 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
- Uchida M et al. Intranasal administration of milnacipran in rats: evaluation of the transport of drugs to the systemic circulation and central nervous system and the pharmacological effect. Biol Pharm Bull 34:740-7 (2011). Read more (PubMed: 21532166) »
- Puozzo C et al. Pharmacology and pharmacokinetics of milnacipran. Int Clin Psychopharmacol 17 Suppl 1:S25-35 (2002). Read more (PubMed: 12369608) »
- Moret C et al. Biochemical profile of midalcipran (F 2207), 1-phenyl-1-diethyl-aminocarbonyl-2-aminomethyl-cyclopropane (Z) hydrochloride, a potential fourth generation antidepressant drug. Neuropharmacology 24:1211-9 (1985). Read more (PubMed: 3005901) »