Recombinant
RabMAb

Recombinant Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)

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Overview

  • Product name

    Anti-Mimitin antibody [EP15279] - C-terminal
    See all Mimitin primary antibodies

  • Description

    Rabbit monoclonal [EP15279] to Mimitin - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Mimitin aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8N183

  • Positive control

    • HepG2 cells and cell lysates: HeLa, SH-SY5Y and HEL cell lysates: Human fetal heart, Mouse kidney and Mouse heart lysates: Human liver and Mouse liver tissue.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IP 1/60.
WB 1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a molecular chaperone for mitochondrial complex I assembly.

  • Tissue specificity

    Highly expressed in ESCC cells. Also expressed in heart, skeletal muscle, liver, and in fibroblasts.

  • Involvement in disease

    Defects in NDUFAF2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.

  • Sequence similarities

    Belongs to the complex I NDUFA12 subunit family.

  • Cellular localization

    Mitochondrion.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • B17.2 like antibody
    • B17.2-like antibody
    • B17.2L antibody
    • FLJ22398 antibody
    • MIMIT_HUMAN antibody
    • Mimitin antibody
    • Mimitin mitochondrial antibody
    • mitochondrial antibody
    • MMTN antibody
    • Myc induced mitochondrial protein antibody
    • Myc-induced mitochondrial protein antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 2 antibody
    • NADH dehydrogenase (ubiquinone) complex I, assembly factor 2 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 antibody
    • NDUFA12 like antibody
    • NDUFA12 like protein antibody
    • NDUFA12-like protein antibody
    • NDUFA12L antibody
    • NDUFAF2 antibody
    • OTTHUMP00000161882 antibody
    • OTTHUMP00000221703 antibody
    see all

Images

  • Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    All lanes : Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : HEL cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/10000 dilution + Human fetal heart lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    All lanes : Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Mimitin with ab192267 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Mimitin with ab192267 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Immunocytochemistry/ Immunofluorescence - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Mimitin with ab192267 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue). 
    Negative controls, ab192267 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.

  • Immunoprecipitation - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
    Immunoprecipitation - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)

    Western blot analysis of Mimitin in immunoprecipitation pellets from MDA-MB-435 lysate. ab192267 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.

References

ab192267 has not yet been referenced specifically in any publications.

Publishing research using ab192267? Please let us know so that we can cite the reference in this datasheet.

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