Recombinant Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP15279] to Mimitin - C-terminal
- Suitable for: ICC/IF, IP, WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Mimitin antibody [EP15279] - C-terminal
See all Mimitin primary antibodies -
Description
Rabbit monoclonal [EP15279] to Mimitin - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2 cells and cell lysates: HeLa, SH-SY5Y and HEL cell lysates: Human fetal heart, Mouse kidney and Mouse heart lysates: Human liver and Mouse liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP15279 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
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IP |
1/60.
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WB |
1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
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IHC-P |
1/100 - 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
1/100. |
IP
1/60. |
WB
1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Acts as a molecular chaperone for mitochondrial complex I assembly. -
Tissue specificity
Highly expressed in ESCC cells. Also expressed in heart, skeletal muscle, liver, and in fibroblasts. -
Involvement in disease
Defects in NDUFAF2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
Sequence similarities
Belongs to the complex I NDUFA12 subunit family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 91942 Human
- Entrez Gene: 75597 Mouse
- Entrez Gene: 361894 Rat
- Omim: 609653 Human
- SwissProt: Q8N183 Human
- SwissProt: Q59J78 Mouse
- Unigene: 591757 Human
- Unigene: 276040 Mouse
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Alternative names
- B17.2 like antibody
- B17.2-like antibody
- B17.2L antibody
see all
Images
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All lanes : Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HEL cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/10000 dilution + Human fetal heart lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa -
All lanes : Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Mimitin with ab192267 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Mimitin with ab192267 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Mimitin with ab192267 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue).
Negative controls, ab192267 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution. -
Western blot analysis of Mimitin in immunoprecipitation pellets from MDA-MB-435 lysate. ab192267 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab192267 has not yet been referenced specifically in any publications.