Recombinant Anti-ROX antibody [EPR11721] (ab173573)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11721] to ROX
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-ROX antibody [EPR11721]
See all ROX primary antibodies -
Description
Rabbit monoclonal [EPR11721] to ROX -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- A431, Raji, Jurkat and HeLa whole cell lysate (ab150035). A431 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11721 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
|
|
ICC/IF |
1/100 - 1/250.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa. |
ICC/IF
1/100 - 1/250. |
Target
-
Function
Involved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. -
Tissue specificity
Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines. -
Sequence similarities
Belongs to the MINA53/NO66 family.
Contains 1 JmjC domain. -
Cellular localization
Nucleus. Nucleus > nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 540466 Cow
- Entrez Gene: 84864 Human
- Entrez Gene: 67014 Mouse
- Entrez Gene: 100174658 Orangutan
- Omim: 612049 Human
- SwissProt: Q5EA24 Cow
- SwissProt: Q8IUF8 Human
- SwissProt: Q8CD15 Mouse
see all -
Alternative names
- 60S ribosomal protein L27a histidine hydroxylase antibody
- Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
- FLJ14393 antibody
see all
Images
-
All lanes : Anti-ROX antibody [EPR11721] (ab173573) at 1/1000 dilution
Lane 1 : A431 cell lysates
Lane 2 : Raji cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 53 kDa -
Immunofluorescence analysis of A431 cells labeling ROX with ab173573 at a 1/100 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab173573 has been referenced in 1 publication.
- Wang Q et al. MDIG promotes cisplatin resistance of lung adenocarcinoma by regulating ABC transporter expression via activation of the WNT/ß-catenin signaling pathway. Oncol Lett 18:4294-4307 (2019). PubMed: 31579066