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We are observing bands in Western blots using ab64457 on various cell lines that are slightly bigger than overexpressed fullength MR or the bands observed from mouse and rat cortex. I would very much like to know the peptide sequence that was used for immunisation, it would help us to decipher what we actually are observing using this antibody.
Asked on Oct 06 2011
Thank you for your inquiry. Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information. We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. Several human Mineralocorticoid Receptor isofomes (4) have been described in the literature. Do you know which one(s) the mouse or rat samples may express? • Could you please specify what size are the non-specific bands? Are they lower or higher than the expected size (107 kDa)? Could you attach an image (saved as a jpeg file) to your e-mail so that I can have a look at the bands. • Would you be so kind to inform me how the samples were prepared and what sort of buffer was applied? • If you wish, I could certainly take a look at the Western blot protocol to see if any tips or recommendations can be made to improve the signal. It would be much appreciated if you could complete the following form (attached as a word document). Thank you for your understanding and co-operation in this matter. I look forward to hearing from you.
Answered on Oct 06 2011