Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)
- Datasheet
- References (17)
- Protocols
Overview
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Product nameAnti-Mineralocorticoid Receptor antibody [H10E4C9F]
See all Mineralocorticoid Receptor primary antibodies -
DescriptionMouse monoclonal [H10E4C9F] to Mineralocorticoid Receptor
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Host speciesMouse
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SpecificityDetects mineralocorticoid receptor (MR).
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Tested applicationsSuitable for: Flow Cyt, Inhibition Assay, ELISA, IHC-P, WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Human
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Immunogen
Aldosterone 3. This antibody was produced using the anti idiotypic method.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberH10E4C9F
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab2774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | 1/20. PubMed: 20573992 ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Inhibition Assay | Use at an assay dependent concentration. ab2774 blocks the binding of aldosterone since it is directed against the steroid binding domain of the mineralocorticoid receptor. |
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| ELISA | Use at an assay dependent concentration. | |
| IHC-P | 1/100. Immunohistochemical staining of MR in rabbit atrium with ab2774 results in strong staining of myocytes and endothelial cells. In immunohistochemical studies, staining with ab2774 is blocked by pre incubating the sample with aldosterone. | |
| WB | 1/250. Predicted molecular weight: 107 kDa. By Western blot, this antibody detects a 116 kDa protein representing MR in rabbit heart homogenate. Due to the extremely low levels of MR expressed in native tissues, it is recommended that enhanced detection systems be used for Western blotting such as enhanced chemiluminescence. | |
| ICC/IF | 1/100. |
Application notes
Is unsuitable for IP.
Target
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FunctionReceptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
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Tissue specificityUbiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.
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Involvement in diseaseDefects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1) [MIM:177735]. PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported.
Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP) [MIM:605115]. Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion. -
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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Post-translational
modificationsPhosphorylated. -
Cellular localizationCytoplasm. Nucleus. Endoplasmic reticulum membrane. Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.
- Information by UniProt
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Database links
- Entrez Gene: 374131 Chicken
- Entrez Gene: 537614 Cow
- Entrez Gene: 475461 Dog
- Entrez Gene: 4306 Human
- Entrez Gene: 110784 Mouse
- Entrez Gene: 25672 Rat
- Omim: 600983 Human
- SwissProt: P08235 Human
see all -
Alternative names
- Aldosterone receptor antibody
- MCR antibody
- MCR_HUMAN antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)](https://www.abcam.com/ps/products/2/ab2774/Images/ab2774-302957-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)Image courtesy of an abreview from Mrs. Barbara Heitkönig.Formalin-fixed, paraffin-embedded cow kidney tissue stained for Mineralocorticoid Receptor using ab2774 at 1/200 dilution in immunohistochemical analysis.
Heat mediated Antigen retrieval using Citrate buffer, 10 mM pH 6.0 was used.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)](https://www.abcam.com/ps/products/2/ab2774/Images/Mineralocorticoid-Receptor-Primary-antibodies-ab2774-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)ab2774 (4µg/ml) staining mineralocorticoid receptor in human pancreas, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the islets of Langerhans and some weaker staining of the exocrine cells of the pancreas.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
![Immunocytochemistry/ Immunofluorescence - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)](https://www.abcam.com/ps/products/2/ab2774/Images/Mineralocorticoid-Receptor-Primary-antibodies-ab2774-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)Image courtesy of an anonymous Abreview.ab2774 staining Mineralocorticoid Receptor in hamster CHO K1 cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and then blocked using TBS, 3% Dried Milk, 0.1 % Triton X-100 for 20 minutes at 22°C. Samples were then incubated with primary antibody at 1/100 for 1 hour at 22°C. The secondary antibody used was a goat anti-mouse IgG conjugated to FITC used at a 1/400 dilution (left hand image). In the right hand image DAPI was used to stain the cell nuclei blue. -
![Flow Cytometry - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)](https://www.abcam.com/ps/products/2/ab2774/Images/Mineralocorticoid-Receptor-Primary-antibodies-ab2774-4.jpg)
Flow Cytometry - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)Overlay histogram showing HEK293 cells stained with ab2774 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2774, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)](https://www.abcam.com/ps/products/2/ab2774/Images/Mineralocorticoid-Receptor-Primary-antibodies-ab2774-7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Mineralocorticoid Receptor ab2774 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)](https://www.abcam.com/ps/products/2/ab2774/Images/Mineralocorticoid-Receptor-Primary-antibodies-ab2774-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Mineralocorticoid Receptor ab2774 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)](https://www.abcam.com/ps/products/2/ab2774/Images/Mineralocorticoid-Receptor-Primary-antibodies-ab2774-9.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human kidney tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Mineralocorticoid Receptor ab2774 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
![Immunocytochemistry/ Immunofluorescence - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)](https://www.abcam.com/ps/products/2/ab2774/Images/Mineralocorticoid-Receptor-Primary-antibodies-ab2774-10.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) ab2774 shows staining in HEK293 cells. Mineralocorticoid Receptor staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor ab2774 at a dilution of 1:20-1:200 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification. -
![Immunocytochemistry/ Immunofluorescence - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)](https://www.abcam.com/ps/products/2/ab2774/Images/Mineralocorticoid-Receptor-Primary-antibodies-ab2774-11.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) ab2774 shows staining in HeLa cells. Mineralocorticoid Receptor staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor ab2774 at a dilution of 1:20-1:200 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification. -
![Immunocytochemistry/ Immunofluorescence - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)](https://www.abcam.com/ps/products/2/ab2774/Images/Mineralocorticoid-Receptor-Primary-antibodies-ab2774-12.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) ab2774 shows staining in HepG2 cells. Mineralocorticoid Receptor staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor ab2774 at a dilution of 1:20-1:200 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody . Images were taken at 60X magnification.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Luo X et al. Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration. Redox Biol 13:508-521 (2017). Read more (PubMed: 28734243) »
- Ghnenis AB et al. Maternal obesity in the ewe increases cardiac ventricular expression of glucocorticoid receptors, proinflammatory cytokines and fibrosis in adult male offspring. PLoS One 12:e0189977 (2017). Read more (PubMed: 29267325) »
