Name: Anti-Mineralocorticoid Receptor antibody [H10E4C9F]
SKU: ab2774
Rating: 4 (5 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)

Key features and details

Mouse monoclonal [H10E4C9F] to Mineralocorticoid Receptor
Suitable for: Flow Cyt, IHC-P, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-Mineralocorticoid Receptor antibody [H10E4C9F]
See all Mineralocorticoid Receptor primary antibodies
Description

Mouse monoclonal [H10E4C9F] to Mineralocorticoid Receptor
Host species

Mouse
Tested applications

Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivity

Reacts with: Human
Immunogen

Aldosterone 3. This antibody was produced using the anti idiotypic method.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

H10E4C9F
Isotype

IgG1
Research areas

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
- Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
Recombinant Protein
- Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		Use at an assay dependent concentration.
IHC-P	(1)	1/100. Immunohistochemical staining of MR in rabbit atrium with ab2774 results in strong staining of myocytes and endothelial cells. In immunohistochemical studies, staining with ab2774 is blocked by pre incubating the sample with aldosterone.
ICC/IF	(1)	1/100.

Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P 1/100. Immunohistochemical staining of MR in rabbit atrium with ab2774 results in strong staining of myocytes and endothelial cells. In immunohistochemical studies, staining with ab2774 is blocked by pre incubating the sample with aldosterone.
ICC/IF 1/100.

Application notes

Is unsuitable for IP.

Function

Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
Tissue specificity

Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.
Involvement in disease

Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1) [MIM:177735]. PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported.
Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP) [MIM:605115]. Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion.
Sequence similarities

Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Post-translational
modifications

Phosphorylated.
Cellular localization

Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.
Target information above from: UniProt accession P08235 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4306 Human
- Omim: 600983 Human
- SwissProt: P08235 Human
- Unigene: 163924 Human
- Unigene: 723668 Human
Alternative names
- Aldosterone receptor antibody
- MCR antibody
- MCR_HUMAN antibody
- MGC133092 antibody
- Mineralocorticoid receptor antibody
- MLR antibody
- MR antibody
- NR3 C2 antibody
- NR3C2 antibody
- NR3C2 protein antibody
- Nuclear receptor subfamily 3 group C member 2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)Image courtesy of an abreview from Mrs. Barbara Heitkönig.

Formalin-fixed, paraffin-embedded cow kidney tissue stained for Mineralocorticoid Receptor using ab2774 at 1/200 dilution in immunohistochemical analysis.

Heat mediated Antigen retrieval using Citrate buffer, 10 mM pH 6.0 was used.
Immunocytochemistry/ Immunofluorescence - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)Image courtesy of an anonymous Abreview.

ab2774 staining Mineralocorticoid Receptor in hamster CHO K1 cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and then blocked using TBS, 3% Dried Milk, 0.1 % Triton X-100 for 20 minutes at 22°C. Samples were then incubated with primary antibody at 1/100 for 1 hour at 22°C. The secondary antibody used was a goat anti-mouse IgG conjugated to FITC used at a 1/400 dilution (left hand image). In the right hand image DAPI was used to stain the cell nuclei blue.
Flow Cytometry - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)

Overlay histogram showing HEK293 cells stained with ab2774 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2774, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)

ab2774 (4µg/ml) staining mineralocorticoid receptor in human pancreas, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the islets of Langerhans and some weaker staining of the exocrine cells of the pancreas.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Mineralocorticoid Receptor ab2774 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Mineralocorticoid Receptor ab2774 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human kidney tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Mineralocorticoid Receptor ab2774 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunocytochemistry/ Immunofluorescence - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)

Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) ab2774 shows staining in HEK293 cells. Mineralocorticoid Receptor staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor ab2774 at a dilution of 1:20-1:200 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)

Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) ab2774 shows staining in HeLa cells. Mineralocorticoid Receptor staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor ab2774 at a dilution of 1:20-1:200 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-Mineralocorticoid Receptor antibody [H10E4C9F] (ab2774)

Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) ab2774 shows staining in HepG2 cells. Mineralocorticoid Receptor staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor ab2774 at a dilution of 1:20-1:200 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody . Images were taken at 60X magnification.

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab2774? Please let us know so that we can cite the reference in this datasheet.

ab2774 has been referenced in 23 publications.

Tanaka S et al. Long-term administration of prednisolone: Effects on the myocardial tissue of healthy beagle dogs. J Vet Med Sci 83:84-93 (2021). IHC-P ; Dog . PubMed: 33268642
Zhang B et al. Corticosteroid receptor rebalancing alleviates critical illness-related corticosteroid insufficiency after traumatic brain injury by promoting paraventricular nuclear cell survival via Akt/CREB/BDNF signaling. J Neuroinflammation 17:318 (2020). PubMed: 33100225
Man AWC et al. Renal Effects of Fetal Reprogramming With Pentaerythritol Tetranitrate in Spontaneously Hypertensive Rats. Front Pharmacol 11:454 (2020). PubMed: 32410988
Wang B et al. The Chronic Adverse Effect of Chloroquine on Kidney in Rats through an Autophagy Dependent and Independent Pathways. Nephron 144:96-108 (2020). PubMed: 31661702
Tata CM et al. Antihypertensive effects of the hydro-ethanol extract of Senecio serratuloides DC in rats. BMC Complement Altern Med 19:52 (2019). PubMed: 30819180

View all Publications for this product

Customer reviews and Q&As

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1-9 of 9 Abreviews or Q&A

Application

IHC - Wholemount

Sample

Dog Tissue (Myocardium, Aorta)

Specification

Myocardium, Aorta

祥代田中

Verified customer

Submitted May 17 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Cow Tissue sections (Kidney)

Specification

Kidney

Fixative

Formaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citratbuffer, 10 mM pH 6,0

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C

Mrs. Barbara Heitkönig

Verified customer

Submitted Jul 07 2011

Application

Western blot

Sample

Mouse Cell lysate - whole cell (kidney fibroblast)

Loading amount

3 µg

Specification

kidney fibroblast

Treatment

aldosterone 5nM 20nM

Gel Running Conditions

Reduced Denaturing (10%)

Blocking step

Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dong Chen

Verified customer

Submitted Sep 02 2009

Application

Immunocytochemistry/ Immunofluorescence

Sample

Hamster Cell (Expression of Human Mineralocorticoid receptor in)

Specification

Expression of Human Mineralocorticoid receptor in

Fixative

Formaldehyde

Blocking step

TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 29 2008

Application

Western blot

Sample

Dog Tissue lysate - whole (left ventricle)

Loading amount

90 µg

Specification

left ventricle

Gel Running Conditions

Reduced Denaturing

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. sudhish mishra

Verified customer

Submitted Jun 19 2007

Question

I am using this antibody in WB of mouse brain tissue lysates and getting no bands. I have loaded 10-60ug of total protein. The membrane is blocked with either BSA or milk. I have tested antibody concentrations 1:1000 - 1:200. Can I try another antibody to this target?

Abcam community

Verified customer

Asked on Jun 15 2012

Answer

Thank you for contacting Abcam regarding ab2774.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.
To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Abcam Scientific Support

Answered on Jun 15 2012

Question

Our lab recently purchased three antibodies (Nitrotyrosine, Mineralocorticoid Receptor and TNF-a) from Abcam. We have stored them at our refrigerator on getting them without checking them out. Today I took them out to make aliquots and noticed the two (MR: ab2774 and TNF-a: ab6671) of them are empty. I do not know what happened on them. As you know they are very expensive. Please let me know what I can do for them.

Abcam community

Verified customer

Asked on Apr 07 2011

Answer

Thank you for contacting us and letting us know about this problem It is possible that the water evaporated from the vials, as both of these antibodies were supplied in small volumes and sometimes the water does evaporate. Ab2774 should contain 100µl of liquid, and ab6771 should contain 40µl. I recommend first spinning the vials down to make sure there is no liquid in the cap. Then, add deionized water until the vials contain the appropriate volumes. If you do have any problems with the functionality of these antibodies, please let me know and I will be happy to send you replacement vials.

Abcam Scientific Support

Answered on Apr 07 2011

Question

I did not order this antibody, I was given an aliquot. The Ab catalog # is ab2774 (H10E4C9G, Mineralcorticoid Receptor) and I would like to know the concentration of the Ab in the 100ul vial that was originally purchased. The data sheet does not contain this information. I do not need to know the concentration of a starting dilution, just the concentration of the Ab itself. For example: 10ug/100ul. I have already contacted Abcam via phone and they did not have access to this information.

Abcam community

Verified customer

Asked on Nov 19 2005

Answer

Thank you for your enquiry. As the purity of ab2774 is ascites, and is therefore unpurified antibody, we cannot provide a concentration. Please contact us again if you have any additional questions.

Abcam Scientific Support

Answered on Nov 21 2005

Question

Some days ago I ordered an antibody from your company. I have recieved the package today. Unfortunatly, the tube you sent me does not contain any liquid material, however, according to the data sheet it should be formulated as 100 microliter liquid, (ascities diluted in PBS). The antibody ordered is the mouse monoclonal [H10E4C9F] one against the mineralocorticoid receptor (ab2774; LOT 58328). Please contact me as soon as possible in order to try to solve this problem.

Abcam community

Verified customer

Asked on Feb 07 2005

Answer

Dear Zoltan, The Mineralocorticoid Receptor ab2774 antibody should have been in liquid form. We do lyophilize some of our products to protect the activity of the antibody during shipment; however we do not show this antibody being lyophilized. Therefore, we will send you a replacement of the ab2774 free of charge. Please let me know if you have any other concerns about any of our quality guaranteed antibodies. Best Wishes,

Abcam Scientific Support

Answered on Feb 08 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Compatible Secondaries

Conjugation kits

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (23)

Customer reviews and Q&As

IHC - Wholemount abreview for Anti-Mineralocorticoid Receptor antibody [H10E4C9F]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mineralocorticoid Receptor antibody [H10E4C9F]

Western blot abreview for Anti-Mineralocorticoid Receptor antibody [H10E4C9F]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Mineralocorticoid Receptor antibody [H10E4C9F]

Western blot abreview for Anti-Mineralocorticoid Receptor antibody [H10E4C9F]

Post-translational
modifications