Overview

  • Product name

    Anti-Mint-1 antibody [EPR15941] - N-terminal
    See all Mint-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15941] to Mint-1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Mint-1 aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: Q02410

  • Positive control

    • Human fetal brain, Human cerebellum, Human Transitional cell carcinoma of bladder.
  • General notes

     

     

    Previously labelled as APBA1. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab190359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 156 kDa (predicted molecular weight: 93 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/40.

Target

  • Function

    Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP.
  • Tissue specificity

    Brain and spinal cord.
  • Sequence similarities

    Contains 2 PDZ (DHR) domains.
    Contains 1 PID domain.
  • Domain

    Composed of an N-terminal domain that binds Munc18-1 and LIN-2/CASK, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adapter protein X11 alpha antibody
    • Adapter protein X11alpha antibody
    • Amyloid beta A4 precursor protein-binding family A member 1 antibody
    • Apba1 antibody
    • APBA1_HUMAN antibody
    • Mint 1 antibody
    • Mint-1 antibody
    • Neuron specific X11 protein antibody
    • Neuron-specific X11 protein antibody
    • Neuronal Munc18 1 interacting protein 1 antibody
    • Neuronal Munc18-1-interacting protein 1 antibody
    • UROP11 antibody
    • x11 antibody
    • X11alpha antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human transitional cell carcinoma of the bladder labeling Mint-1 with ab190359 at 1/50 dilution and HRP polymer for Rabbit IgG. Counterstained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359) at 1/50000 dilution + Human fetal brain at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 93 kDa
    Observed band size: 156 kDa
    why is the actual band size different from the predicted?

  • Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359) at 1/10000 dilution + Human cerebellum at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 93 kDa
    Observed band size: 156 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation of Human fetal brain labeling Mint-1 with ab190359 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000

References

ab190359 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab190359.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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