Key features and details
- Rabbit polyclonal to Mint-2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Mint-2 antibody
DescriptionRabbit polyclonal to Mint-2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cat, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
Synthetic peptide within Human Mint-2 aa 125-175. The exact sequence is proprietary.
Database link: Q99767
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysates.
Previously labelled as APBA2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226833 was affinity purified using an epitope specific to Mint-2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 82 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPutative function in synaptic vesicle exocytosis by binding to STXBP1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP.
Sequence similaritiesContains 2 PDZ (DHR) domains.
Contains 1 PID domain.
DomainComposed of an N-terminal domain that binds STXBP1, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.
- Information by UniProt
- Adapter protein X11beta antibody
- Amyloid beta A4 precursor protein-binding family A member 2 antibody
- APBA2 antibody
All lanes : Anti-Mint-2 antibody (ab226833) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 82 kDa
Exposure time: 30 seconds
Lysates were prepared using NETN lysis buffer.
Mint-2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226833 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226833 at 0.4 µg/ml.
Lane 1: ab226833 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226833 has not yet been referenced specifically in any publications.