Product nameAnti-Mint-2 antibody
See all Mint-2 primary antibodies
DescriptionRabbit polyclonal to Mint-2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cat, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
Synthetic peptide within Human Mint-2 aa 125-175. The exact sequence is proprietary.
Database link: Q99767
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysates.
Previously labelled as APBA2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226833 was affinity purified using an epitope specific to Mint-2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 82 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPutative function in synaptic vesicle exocytosis by binding to STXBP1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP.
Sequence similaritiesContains 2 PDZ (DHR) domains.
Contains 1 PID domain.
DomainComposed of an N-terminal domain that binds STXBP1, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.
- Information by UniProt
- Adapter protein X11beta antibody
- Amyloid beta A4 precursor protein-binding family A member 2 antibody
- APBA2 antibody
All lanes : Anti-Mint-2 antibody (ab226833) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 82 kDa
Exposure time: 30 seconds
Lysates were prepared using NETN lysis buffer.
Mint-2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226833 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226833 at 0.4 µg/ml.
Lane 1: ab226833 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab226833 has not yet been referenced specifically in any publications.