Overview

  • Product name

  • Description

    Rabbit polyclonal to Mint-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cat, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide within Human Mint-2 aa 125-175. The exact sequence is proprietary.
    Database link: Q99767

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysates.
  • General notes

    Previously labelled as APBA2.

Properties

Applications

Our Abpromise guarantee covers the use of ab226833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 82 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Putative function in synaptic vesicle exocytosis by binding to STXBP1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP.
  • Tissue specificity

    Brain.
  • Sequence similarities

    Contains 2 PDZ (DHR) domains.
    Contains 1 PID domain.
  • Domain

    Composed of an N-terminal domain that binds STXBP1, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adapter protein X11beta antibody
    • Amyloid beta A4 precursor protein-binding family A member 2 antibody
    • APBA2 antibody
    • APBA2_HUMAN antibody
    • D15S1518E antibody
    • HsT16821 antibody
    • LIN 10 antibody
    • MGC:14091 antibody
    • MGC99508 antibody
    • Mint 2 antibody
    • Mint-2 antibody
    • MINT2 antibody
    • Neuron-specific X11L protein antibody
    • Neuronal Munc18-1-interacting protein 2 antibody
    • Phosphotyrosine binding/interacting domain (PTB) bearing protein antibody
    • X11 beta antibody
    • X11 like protein antibody
    • X11l antibody
    see all

Images

  • All lanes : Anti-Mint-2 antibody (ab226833) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 82 kDa


    Exposure time: 30 seconds


    Lysates were prepared using NETN lysis buffer.

  • Mint-2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226833 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226833 at 0.4 µg/ml.

    Lane 1: ab226833 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab226833 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226833.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up