Anti-Mint3 antibody (ab3450)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Mint3 antibody
See all Mint3 primary antibodies -
DescriptionRabbit polyclonal to Mint3
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to Rat Mint3 aa 1-19.
Sequence:MEFLPEPQHPPGPPTMDLE
(Peptide available asab4981) -
Positive control
- AtT20 cell extract.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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PurityImmunogen affinity purified
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Primary antibody notesThe munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to munc-18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint 3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint 3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab3450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | Use at an assay dependent concentration. | |
WB | Use a concentration of 2 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 61 kDa).Can be blocked with Mint3 peptide (ab4981). By Western blot, this antibody detects an ~86 kDa protein representing Mint 3 from AtT20 cell extract. |
Target
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FunctionMay modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP. May enhance the activity of HIF1A in macrophages by inhibiting the activity of HIF1AN.
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Tissue specificityExpressed in all tissues examined with lower levels in brain and testis.
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Sequence similaritiesContains 2 PDZ (DHR) domains.
Contains 1 PID domain. -
DomainComposed of an N-terminal domain, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.
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Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
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Database links
- Entrez Gene: 9546 Human
- Entrez Gene: 57267 Mouse
- Entrez Gene: 83611 Rat
- Omim: 604262 Human
- SwissProt: O96018 Human
- SwissProt: O88888 Mouse
- SwissProt: O70248 Rat
- Unigene: 25527 Human
see all -
Alternative names
- Adapter protein X11gamma antibody
- amyloid beta (A4) precursor protein binding, family A, member 3 antibody
- Amyloid beta A4 precursor protein-binding family A member 3 antibody
see all
Images
Datasheets and documents
References
ab3450 has not yet been referenced specifically in any publications.