Overview

  • Product name
    Anti-Mint3 antibody
    See all Mint3 primary antibodies
  • Description
    Rabbit polyclonal to Mint3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat Mint3 aa 1-19.
    Sequence:

    MEFLPEPQHPPGPPTMDLE


    (Peptide available as ab4981)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • AtT20 cell extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to munc-18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint 3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint 3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 61 kDa).Can be blocked with Mint3 peptide (ab4981).

By Western blot, this antibody detects an ~86 kDa protein representing Mint 3 from AtT20 cell extract.

Target

  • Function
    May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP. May enhance the activity of HIF1A in macrophages by inhibiting the activity of HIF1AN.
  • Tissue specificity
    Expressed in all tissues examined with lower levels in brain and testis.
  • Sequence similarities
    Contains 2 PDZ (DHR) domains.
    Contains 1 PID domain.
  • Domain
    Composed of an N-terminal domain, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Adapter protein X11gamma antibody
    • amyloid beta (A4) precursor protein binding, family A, member 3 antibody
    • Amyloid beta A4 precursor protein-binding family A member 3 antibody
    • Amyloid beta precursor protein binding, family A, member 3 phosphotyrosine binding/ interacting domain (PTB) bearing protein antibody
    • APBA3 antibody
    • APBA3_HUMAN antibody
    • lin 10 antibody
    • MGC:15815 antibody
    • Mint 3 antibody
    • Mint-3 antibody
    • Neuron specific X11L2 protein antibody
    • Neuron-specific X11L2 protein antibody
    • Neuronal Munc18 1 interacting protein 3 antibody
    • Neuronal Munc18-1-interacting protein 3 antibody
    • phosphotyrosine binding/interacting domain (PTB) bearing protein antibody
    • X11 like 2 protein 2 antibody
    • X11gamma antibody
    • X11L2 antibody
    see all

Images

  • Western blot - Anti-Mint3 antibody (ab3450)
    Western blot - Anti-Mint3 antibody (ab3450)

    Western blot detection of Mint3 on AtT20 cell extract using ab3450.

References

ab3450 has not yet been referenced specifically in any publications.

Publishing research using ab3450? Please let us know so that we can cite the reference in this datasheet.

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Mint3 antibody

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Specification
MEF
Fixative
Methanol/Acetone 50:50
Permeabilization
Yes - PBS, Triton X-100 (0.1%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21°C
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Submitted Jul 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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