Anti-Mint3 antibody (ab3450)
Key features and details
- Rabbit polyclonal to Mint3
- Suitable for: WB, ICC
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Mint3 antibody -
Description
Rabbit polyclonal to Mint3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Rat Mint3 aa 1-19.
Sequence:MEFLPEPQHPPGPPTMDLE
(Peptide available asab4981) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to munc-18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint 3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint 3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 61 kDa).
By Western blot, this antibody detects an ~86 kDa protein representing Mint 3 from AtT20 cell extract. |
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| ICC |
Use a concentration of 2 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 61 kDa). By Western blot, this antibody detects an ~86 kDa protein representing Mint 3 from AtT20 cell extract. |
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ICC
Use a concentration of 2 µg/ml. |
Target
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Function
May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP. May enhance the activity of HIF1A in macrophages by inhibiting the activity of HIF1AN. -
Tissue specificity
Expressed in all tissues examined with lower levels in brain and testis. -
Sequence similarities
Contains 2 PDZ (DHR) domains.
Contains 1 PID domain. -
Domain
Composed of an N-terminal domain, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 57267 Mouse
- SwissProt: O88888 Mouse
- Unigene: 293931 Mouse
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Alternative names
- Adapter protein X11gamma antibody
- amyloid beta (A4) precursor protein binding, family A, member 3 antibody
- Amyloid beta A4 precursor protein-binding family A member 3 antibody
see all
Images
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Western blot - Anti-Mint3 antibody (ab3450)
Western blot detection of Mint3 on AtT20 cell extract using ab3450.
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Immunocytochemistry - Anti-Mint3 antibody (ab3450)Neuro-2a cells stained for Mint3 (green) using ab3450 at 2 µg/mL in immunocytochemical analysis. Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab3450 has not yet been referenced specifically in any publications.
