Anti-Mint3 antibody (ab3450)

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Western blot - Anti-Mint3 antibody (ab3450)

    Key features and details

    • Rabbit polyclonal to Mint3
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    Overview

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Primary antibody notes

      The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to munc-18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint 3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint 3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3450 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    Application Abreviews Notes
    WB
    Use a concentration of 2 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 61 kDa).

    By Western blot, this antibody detects an ~86 kDa protein representing Mint 3 from AtT20 cell extract.
    Notes
    WB
    Use a concentration of 2 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 61 kDa).

    By Western blot, this antibody detects an ~86 kDa protein representing Mint 3 from AtT20 cell extract.

    Target

    • Function

      May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP. May enhance the activity of HIF1A in macrophages by inhibiting the activity of HIF1AN.

    • Tissue specificity

      Expressed in all tissues examined with lower levels in brain and testis.

    • Sequence similarities

      Contains 2 PDZ (DHR) domains.
      Contains 1 PID domain.

    • Domain

      Composed of an N-terminal domain, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.

    • Cellular localization

      Cytoplasm > perinuclear region.
    • Information by UniProt

    • Database links

      • Alternative names

        • Adapter protein X11gamma antibody
        • amyloid beta (A4) precursor protein binding, family A, member 3 antibody
        • Amyloid beta A4 precursor protein-binding family A member 3 antibody
        • Amyloid beta precursor protein binding, family A, member 3 phosphotyrosine binding/ interacting domain (PTB) bearing protein antibody
        • APBA3 antibody
        • APBA3_HUMAN antibody
        • lin 10 antibody
        • MGC:15815 antibody
        • Mint 3 antibody
        • Mint-3 antibody
        • Neuron specific X11L2 protein antibody
        • Neuron-specific X11L2 protein antibody
        • Neuronal Munc18 1 interacting protein 3 antibody
        • Neuronal Munc18-1-interacting protein 3 antibody
        • phosphotyrosine binding/interacting domain (PTB) bearing protein antibody
        • X11 like 2 protein 2 antibody
        • X11gamma antibody
        • X11L2 antibody
        see all

      Images

      • Western blot - Anti-Mint3 antibody (ab3450)
        Western blot - Anti-Mint3 antibody (ab3450)

        Western blot detection of Mint3 on AtT20 cell extract using ab3450.

      References (0)

      Publishing research using ab3450? Please let us know so that we can cite the reference in this datasheet.

      ab3450 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-Mint3 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (MEF)
      Specification
      MEF
      Fixative
      Methanol/Acetone 50:50
      Permeabilization
      Yes - PBS, Triton X-100 (0.1%)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21°C
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      Verified customer

      Submitted Jul 06 2010

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