Product nameAnti-MIOX antibody [EPR17173]
See all MIOX primary antibodies
DescriptionRabbit monoclonal [EPR17173] to MIOX
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MIOX aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UGB7
- WB: Human fetal kidney lysate. IP: Human fetal kidney whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab198997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
Tissue specificityKidney specific.
PathwayPolyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.
Sequence similaritiesBelongs to the myo-inositol oxygenase family.
- Information by UniProt
- 0610009I10Rik antibody
- AI314022 antibody
- Aldehyde reductase-like 6 antibody
All lanes : Anti-MIOX antibody [EPR17173] (ab198997) at 1/2000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 10 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
MIOX is specifically expressed in the kidney. Lane 2, Human fetal heart, represents a negative control.
MIOX was immunoprecipitated from 1mg of Human fetal kidney whole cell extract with ab198997 at 1/150 dilution. Western blot was performed using ab198997 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal kidney whole cell extract 10µg (Input).
Lane 2: ab198997 IP in Human fetal kidney whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198997 in Human fetal kidney whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
ab198997 has not yet been referenced specifically in any publications.