Recombinant Anti-MIOX antibody [EPR17173] (ab198997)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17173] to MIOX
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-MIOX antibody [EPR17173]
See all MIOX primary antibodies -
Description
Rabbit monoclonal [EPR17173] to MIOX -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MIOX aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UGB7 -
Positive control
- WB: Human fetal kidney lysate. IP: Human fetal kidney whole cell extract.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17173 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab198997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/150. | |
| WB | 1/2000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
Target
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Tissue specificity
Kidney specific. -
Pathway
Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1. -
Sequence similarities
Belongs to the myo-inositol oxygenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55586 Human
- Omim: 606774 Human
- SwissProt: Q9UGB7 Human
- Unigene: 129227 Human
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Alternative names
- 0610009I10Rik antibody
- AI314022 antibody
- Aldehyde reductase-like 6 antibody
see all
Images
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![Western blot - Anti-MIOX antibody [EPR17173] (ab198997)](https://www.abcam.com/ps/products/198/ab198997/Images/ab198997-241822-anti-miox-antibody-epr17173-western-blot.jpg)
Western blot - Anti-MIOX antibody [EPR17173] (ab198997)All lanes : Anti-MIOX antibody [EPR17173] (ab198997) at 1/2000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
MIOX is specifically expressed in the kidney. Lane 2, Human fetal heart, represents a negative control.
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![Immunoprecipitation - Anti-MIOX antibody [EPR17173] (ab198997)](https://www.abcam.com/ps/products/198/ab198997/Images/ab198997-241823-anti-miox-antibody-epr17173-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MIOX antibody [EPR17173] (ab198997)MIOX was immunoprecipitated from 1mg of Human fetal kidney whole cell extract with ab198997 at 1/150 dilution. Western blot was performed using ab198997 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal kidney whole cell extract 10µg (Input).
Lane 2: ab198997 IP in Human fetal kidney whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198997 in Human fetal kidney whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-MIOX antibody [EPR17173] (ab198997)](https://www.abcam.com/ps/products/198/ab198997/Images/ab198997-3-benefits-of-recombinant-antibodies.png)
Anti-MIOX antibody [EPR17173] (ab198997)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab198997 has not yet been referenced specifically in any publications.
