Recombinant Anti-MIOX antibody [EPR17173] (ab198997)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MIOX antibody [EPR17173]
See all MIOX primary antibodies -
Description
Rabbit monoclonal [EPR17173] to MIOX -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MIOX aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UGB7 -
Positive control
- WB: Human fetal kidney lysate. IP: Human fetal kidney whole cell extract.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17173 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab198997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/150. | |
| WB | 1/2000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
Target
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Tissue specificity
Kidney specific. -
Pathway
Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1. -
Sequence similarities
Belongs to the myo-inositol oxygenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55586 Human
- Omim: 606774 Human
- SwissProt: Q9UGB7 Human
- Unigene: 129227 Human
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Alternative names
- 0610009I10Rik antibody
- AI314022 antibody
- Aldehyde reductase-like 6 antibody
see all
Images
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![Western blot - Anti-MIOX antibody [EPR17173] (ab198997)](https://www.abcam.com/ps/products/198/ab198997/Images/ab198997-241822-anti-miox-antibody-epr17173-western-blot.jpg)
Western blot - Anti-MIOX antibody [EPR17173] (ab198997)All lanes : Anti-MIOX antibody [EPR17173] (ab198997) at 1/2000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
MIOX is specifically expressed in the kidney. Lane 2, Human fetal heart, represents a negative control.
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![Immunoprecipitation - Anti-MIOX antibody [EPR17173] (ab198997)](https://www.abcam.com/ps/products/198/ab198997/Images/ab198997-241823-anti-miox-antibody-epr17173-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MIOX antibody [EPR17173] (ab198997)MIOX was immunoprecipitated from 1mg of Human fetal kidney whole cell extract with ab198997 at 1/150 dilution. Western blot was performed using ab198997 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal kidney whole cell extract 10µg (Input).
Lane 2: ab198997 IP in Human fetal kidney whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198997 in Human fetal kidney whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Datasheets and documents
References
ab198997 has not yet been referenced specifically in any publications.
