Recombinant
RabMAb

Recombinant Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877)

Overview

  • Product name

    Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)]
    See all MIP-3 beta/CCL19 primary antibodies
  • Description

    Rabbit monoclonal [EPR7044(2)] to MIP-3 beta/CCL19
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human MIP-3 beta/CCL19 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q99731

  • Positive control

    • A549 cells; Human MIP-3 beta/CCL19 recombinant protein (aa22-98).
  • General notes

     

     

     This product was previously labelled as Macrophage Inflammatory Protein 3 beta

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
Flow Cyt 1/170.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes.
  • Tissue specificity

    Expressed at high levels in the lymph nodes, thymus and appendix. Intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta chemokine exodus 3 antibody
    • Beta-chemokine exodus-3 antibody
    • C C chemokine ligand 19 antibody
    • C-C motif chemokine 19 antibody
    • CC chemokine ligand 19 antibody
    • CCL 19 antibody
    • CCL19 antibody
    • CCL19_HUMAN antibody
    • Chemokine (C C motif) ligand 19 antibody
    • Chemokine (CC motif) ligand 19 antibody
    • Chemokine C C Motif Ligand 19 antibody
    • Chemokine CC Motif Ligand 19 antibody
    • CK beta 11 antibody
    • CK beta-11 antibody
    • CKb 11 antibody
    • CKb11 antibody
    • EBI 1 ligand chemokine antibody
    • EBI1 ligand chemokine antibody
    • ELC antibody
    • Epstein-Barr virus-induced molecule 1 ligand chemokine antibody
    • Exodus 3 antibody
    • Exodus3 antibody
    • Macrophage inflammatory protein 3 beta antibody
    • MGC34433 antibody
    • MIP 3 beta antibody
    • MIP 3B antibody
    • MIP-3-beta antibody
    • MIP-3b antibody
    • MIP3 beta antibody
    • MIP3B antibody
    • OTTHUMP00000000531 antibody
    • SCYA 19 antibody
    • SCYA19 antibody
    • Small inducible cytokine A19 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 1 antibody
    • Small-inducible cytokine A19 antibody
    see all

Images

  • Flow cytometric analysis of 2% paraformaldehyde-fixed A549 cells labeling MIP-3 beta/CCL19 with ab192877 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877) at 1/20000 dilution + Human MIP-3 beta/CCL19 recombinant protein (aa22-98) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking/Dilution buffer: 5% NFDM /TBST.

References

ab192877 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192877.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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