Overview

  • Product name

    Anti-MIPEP antibody
  • Description

    Rabbit polyclonal to MIPEP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 180-396 of Human MIPEP (UniProt ID: Q99797).

  • Positive control

    • A431 and HepG2 whole cell lysate (ab7900); A431 cells; Human CL15 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 81 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Cleaves proteins, imported into the mitochondrion, to their mature size.
  • Sequence similarities

    Belongs to the peptidase M3 family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 3.4.24.59 antibody
    • HMIP antibody
    • MIP antibody
    • Mipep antibody
    • MIPEP_HUMAN antibody
    • Mitochondrial intermediate peptidase antibody
    • Mitochondrial intermedieate peptide antibody
    • OTTHUMP00000018121 antibody
    • OTTHUMP00000042292 antibody
    see all

Images

  • Anti-MIPEP antibody (ab154407) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 81 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed A431 cells, labeling MIPEP using ab154407 at a 1/200 dilution.
    The image in the lower panel was co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded Human CL15 xenograft tissue, labeling MIPEP using ab154407 at a 1/100 dilution.

References

ab154407 has not yet been referenced specifically in any publications.

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