Key features and details
- Rabbit polyclonal to MIPEP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MIPEP antibody
DescriptionRabbit polyclonal to MIPEP
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to a region within amino acids 180-396 of Human MIPEP (UniProt ID: Q99797).
- A431 and HepG2 whole cell lysate (ab7900); A431 cells; Human CL15 xenograft tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 81 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionCleaves proteins, imported into the mitochondrion, to their mature size.
Sequence similaritiesBelongs to the peptidase M3 family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- EC 22.214.171.124 antibody
- HMIP antibody
- MIP antibody
Anti-MIPEP antibody (ab154407) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 81 kDa
7.5% SDS PAGE
Immunofluorescence analysis of methanol-fixed A431 cells, labeling MIPEP using ab154407 at a 1/200 dilution.
The image in the lower panel was co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin embedded Human CL15 xenograft tissue, labeling MIPEP using ab154407 at a 1/100 dilution.
ab154407 has not yet been referenced specifically in any publications.