Anti-Miranda antibody [CD#5- 7E9BG5AF4] (ab197788)

Overview

  • Product name

    Anti-Miranda antibody [CD#5- 7E9BG5AF4]
  • Description

    Rat monoclonal [CD#5- 7E9BG5AF4] to Miranda
  • Host species

    Rat
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purification notes

    Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    CD#5- 7E9BG5AF4
  • Isotype

    IgG2b

Applications

Our Abpromise guarantee covers the use of ab197788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Database links

    • Alternative names

      • CG12249 antibody
      • Dmel\CG12249 antibody
      • Mir antibody
      • mira antibody

    Images

    • Immunohistochemistry analysis using Miranda rat monoclonal to detect Miranda protein in all neuroblasts from Drosophila stage 10 embryos. Lateral view showing asymmetric partitioning of Miranda protein to the basal cortex of the neuroblasts, and then specifically into the smaller basal daughter cells (GMCs). The Miranda antibody was used at 3.4 µg/mL and detected with Alexa488 conjugated goat anti-rat at 1:400. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum.

    References

    This product has been referenced in:

    See all 5 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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