Anti-Miranda antibody [CD#5- 7E9BG5AF4] (ab197788)

Overview

  • Product name
    Anti-Miranda antibody [CD#5- 7E9BG5AF4]
  • Description
    Rat monoclonal [CD#5- 7E9BG5AF4] to Miranda
  • Host species
    Rat
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to Fruit fly (Drosophila melanogaster) Miranda conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9VDR7

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Clonality
    Monoclonal
  • Clone number
    CD#5- 7E9BG5AF4
  • Isotype
    IgG2b

Applications

Our Abpromise guarantee covers the use of ab197788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Database links
    • Alternative names
      • CG12249 antibody
      • Dmel\CG12249 antibody
      • Mir antibody
      • mira antibody

    Images

    • Immunohistochemistry analysis using Miranda rat monoclonal to detect Miranda protein in all neuroblasts from Drosophila stage 10 embryos. Lateral view showing asymmetric partitioning of Miranda protein to the basal cortex of the neuroblasts, and then specifically into the smaller basal daughter cells (GMCs). The Miranda antibody was used at 3.4 µg/mL and detected with Alexa488 conjugated goat anti-rat at 1:400. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum.

    References

    This product has been referenced in:
    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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