Product nameAnti-MIRO2 antibody
See all MIRO2 primary antibodies
DescriptionRabbit polyclonal to MIRO2
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Mouse MIRO2 aa 400-500 conjugated to keyhole limpet haemocyanin.
Database link: Q8JZN7
- This antibody gave a positive signal in the following Mouse tissues; Heart; Skeletal Muscle; Kidney; Pancreas. This antibody gave a positive result when used in the following methanol fixed cell lines: Hek293.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa).|
FunctionMitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
Tissue specificityUbiquitously expressed. Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the mitochondrial Rho GTPase family.
Contains 2 EF-hand domains.
Contains 2 Miro domains.
Cellular localizationMitochondrion outer membrane. Colocalizes with MGARP and RHOT2 at the mitochondria.
- Information by UniProt
- ARHT2 antibody
- C16orf39 antibody
- hMiro 2 antibody
ICC/IF image of ab154946 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab154946 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-MIRO2 antibody (ab154946) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lane 4 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa (possible non-specific binding)
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab154946 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406