• Product name

  • Description

    Rabbit polyclonal to MIRO2
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse MIRO2 aa 400-500 conjugated to keyhole limpet haemocyanin.
    Database link: Q8JZN7

  • Positive control

    • This antibody gave a positive signal in the following Mouse tissues; Heart; Skeletal Muscle; Kidney; Pancreas. This antibody gave a positive result when used in the following methanol fixed cell lines: Hek293.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab154946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa).


  • Function

    Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
  • Tissue specificity

    Ubiquitously expressed. Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the mitochondrial Rho GTPase family.
    Contains 2 EF-hand domains.
    Contains 2 Miro domains.
  • Cellular localization

    Mitochondrion outer membrane. Colocalizes with MGARP and RHOT2 at the mitochondria.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARHT2 antibody
    • C16orf39 antibody
    • hMiro 2 antibody
    • hMiro-2 antibody
    • hMiro2 antibody
    • MIRO-2 antibody
    • MIRO2_HUMAN antibody
    • Mitochondrial Rho GTPase 2 antibody
    • Ras homolog gene family member T2 antibody
    • RASL antibody
    • RHOT2 antibody
    see all


  • ICC/IF image of ab154946 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab154946 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-MIRO2 antibody (ab154946) at 1 µg/ml (Milk blocking 3%)

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Kidney (Mouse) Tissue Lysate
    Lane 4 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 68 kDa
    Observed band size: 74 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 36 kDa (possible non-specific binding)

    Exposure time: 8 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab154946 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406


This product has been referenced in:

  • Park S  et al. UbMES and UbFluor: Novel probes for ring-between-ring (RBR) E3 ubiquitin ligase PARKIN. J Biol Chem 292:16539-16553 (2017). Read more (PubMed: 28710279) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154946.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up