Key features and details
- Rabbit polyclonal to MIRO2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MIRO2 antibody
See all MIRO2 primary antibodies
DescriptionRabbit polyclonal to MIRO2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- WB: HEK-293 cell lysate. IHC-P: Human small intestine tissue. ICC/IF: U-2 OS cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 23,68 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionMitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
Tissue specificityUbiquitously expressed. Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the mitochondrial Rho GTPase family.
Contains 2 EF-hand domains.
Contains 2 Miro domains.
Cellular localizationMitochondrion outer membrane. Colocalizes with MGARP and RHOT2 at the mitochondria.
- Information by UniProt
- ARHT2 antibody
- C16orf39 antibody
- hMiro 2 antibody
Anti-MIRO2 antibody (ab224089) at 1/100 dilution + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 23,68 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for MIRO2 (green) using ab224089 at 4 µg/ml in ICC/IF.
Paraffin-embedded human small intestine tissue stained for MIRO2 using ab224089 at 1/50 dilution in immunohistochemical analysis.
ab224089 has not yet been referenced specifically in any publications.