Overview

  • Product name

  • Description

    Mouse polyclonal to MIRO2
  • Host species

    Mouse
  • Specificity

    This antibody has been tested with MIRO2 transfected 293T cell lysate. We have no data on endogenous samples.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human MIRO2.
    Database link: Q8IXI1

  • Positive control

    • MIRO2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa.

Target

  • Function

    Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
  • Tissue specificity

    Ubiquitously expressed. Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the mitochondrial Rho GTPase family.
    Contains 2 EF-hand domains.
    Contains 2 Miro domains.
  • Cellular localization

    Mitochondrion outer membrane. Colocalizes with MGARP and RHOT2 at the mitochondria.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARHT2 antibody
    • C16orf39 antibody
    • hMiro 2 antibody
    • hMiro-2 antibody
    • hMiro2 antibody
    • MIRO-2 antibody
    • MIRO2_HUMAN antibody
    • Mitochondrial Rho GTPase 2 antibody
    • Ras homolog gene family member T2 antibody
    • RASL antibody
    • RHOT2 antibody
    see all

Images

  • All lanes : Anti-MIRO2 antibody (ab67588) at 1/500 dilution

    Lane 1 : RHOT2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Observed band size: 68 kDa
    why is the actual band size different from the predicted?

References

ab67588 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Q&A

Answer

Thank you very much for sending the image.

Please keep me updated about the results with the replacement antibody, and let me know if there is anything else that we can do for you.

Have a nice day.

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Answer

Thank you for your call today and for letting us know about the trouble with ab67588. I apologize again for the previous confusion regarding the expected molecular weight of this protein.

As we discussed, I'm sending a free of charge vial of ab24710 on the order ***, which will ship out on Monday and should arrive Tuesday or Wednesday. Please keep me updated about the results with this replacement antibody.

You can also reply to this email and attach the image of the blot using ab67588, which will be very useful for us.

If there is anything else that we can do for you, please don't hesitate to ask. Have a great day and a wonderful weekend.

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Answer

Thank you for your inquiry.

I was in touch with the lab and they confirmed that they did not test this product in endogenous samples, only RHOT2 transfected 293T cell lysate.

However, they were able to update us with a new WB image from their testing. This testing is more in line with what we would expect. The band is seen around 70 - 75 kDa. I've updated our datasheet, but this usually takes a few days to go live, so I have also attached the new image.

The expected MW if 68 kDa, based on the SwissProt information.

http://www.uniprot.org/uniprot/Q8IXI1

Based on this and the new data from the lab, I would believe that the band you are seeing is the protein of interest. You may want to submit these findings as an Abreview to earn Abpoints towards Amazon giftcards or discounts on future Abcam orders.

https://www.abcam.com/index.html?pageconfig=resource&rid=10332

I hope this information helps. Please contact us with any other questions.

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Answer

Thank you for bringing this to our attention. I've been in touch with the lab to see if they have any endogenous testing data.
Here is the subcellular fractionation protocol we talked about on the phone.
https://www.abcam.com/index.html?pageconfig=resource&rid=11473
Here are our fractionation kits as well.
https://www.abcam.com/index.html?c=4573
I'll be in touch again once I have more information from the lab.

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