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I'm using this antibody to detect the endogenous protein in cell lysate and seeing a band at about 60 kDa.
Asked on Apr 09 2012
Thank you for your inquiry.
I was in touch with the lab and they confirmed that they did not test this product in endogenous samples, only RHOT2 transfected 293T cell lysate.
However, they were able to update us with a new WB image from their testing. This testing is more in line with what we would expect. The band is seen around 70 - 75 kDa. I've updated our datasheet, but this usually takes a few days to go live, so I have also attached the new image.
The expected MW if 68 kDa, based on the SwissProt information.
Based on this and the new data from the lab, I would believe that the band you are seeing is the protein of interest. You may want to submit these findings as an Abreview to earn Abpoints towards Amazon giftcards or discounts on future Abcam orders.
I hope this information helps. Please contact us with any other questions.
Answered on Apr 09 2012