Overview

  • Product name

  • Description

    Rabbit polyclonal to MIS12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human MIS12 (internal sequence). The exact sequence is proprietary.
    Database link: Q9H081

  • Positive control

    • WB: HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab229337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IP 1/100 - 1/500.

Target

  • Relevance

    MIS12 is part of a complex that plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly.
  • Cellular localization

    Nucleus. Associated with the kinetochore.
  • Database links

  • Alternative names

    • hMIS12 antibody
    • homolog of yeast Mis12 antibody
    • KNTC2AP antibody
    • MIND kinetochore complex component, homolog (yeast) antibody
    • MIS12 homolog (yeast) antibody
    • MIS12 homolog antibody
    • MIS12 MIND kinetochore complex component homolog (yeast) antibody
    • MTW 1 antibody
    • MTW1 antibody
    • Protein MIS12 homolog antibody
    see all

Images

  • MIS12 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg of ab229337. Western blot was performed from the immunoprecipitate using ab229337.

    Lane 1: Control IgG instead of ab229337 in HEK-293T whole cell extract.
    Lane 2: ab229337 IP in HEK-293T whole cell extract.

  • All lanes : Anti-MIS12 antibody (ab229337) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 24 kDa



    12% SDS-PAGE gel.

References

ab229337 has not yet been referenced specifically in any publications.

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