Anti-MIS12 antibody (ab70843)
Key features and details
- Rabbit polyclonal to MIS12
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MIS12 antibody
See all MIS12 primary antibodies -
Description
Rabbit polyclonal to MIS12 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan, Bat
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Immunogen
Synthetic peptide corresponding to a region between residue 150 and the C-terminus (residue 205) of human MIS12. (NP_076944.1)
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab70843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 - 5 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/2500. Detects a band of approximately 34 kDa (predicted molecular weight: 24 kDa). |
Target
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Relevance
MIS12 is part of a complex that plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly. -
Cellular localization
Nucleus. Associated with the kinetochore. -
Database links
- Entrez Gene: 79003 Human
- Omim: 609178 Human
- SwissProt: Q9H081 Human
- Unigene: 267194 Human
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Alternative names
- hMIS12 antibody
- homolog of yeast Mis12 antibody
- KNTC2AP antibody
see all
Images
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Immunoprecipitation - Anti-MIS12 antibody (ab70843)HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70843 at 1 µg/ml for WB, subsequent to being used for IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds. -
Western blot - Anti-MIS12 antibody (ab70843)All lanes : Anti-MIS12 antibody (ab70843) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIS12 antibody (ab70843)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling MIS12 with ab70843 at 1/1000 (1µg/ml). Detection: DAB.
Protocols
References (5)
ab70843 has been referenced in 5 publications.
- Petsalaki E et al. Chmp4c is required for stable kinetochore-microtubule attachments. Chromosoma N/A:N/A (2018). PubMed: 29968190
- Niikura Y et al. SGT1-HSP90 complex is required for CENP-A deposition at centromeres. Cell Cycle 16:1683-1694 (2017). PubMed: 28816574
- Zhou H et al. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun 7:10151 (2016). PubMed: 26743940
- Milev MP et al. TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment. J Cell Biol 209:221-34 (2015). WB, ICC/IF ; Human . PubMed: 25918224
- Petrovic A et al. The MIS12 complex is a protein interaction hub for outer kinetochore assembly. J Cell Biol 190:835-52 (2010). WB ; Human . PubMed: 20819937
