- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-MIS12 antibody
See all MIS12 primary antibodies -
DescriptionRabbit polyclonal to MIS12
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Host speciesRabbit
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Tested applicationsSuitable for: IP, IHC-P, ICC/IF, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan, Bat
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Immunogen
Synthetic peptide corresponding to a region between residue 150 and the C-terminus (residue 205) of human MIS12. (NP_076944.1)
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Positive control
- HeLa and 293T whole cell lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab70843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 - 5 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/2500. Detects a band of approximately 34 kDa (predicted molecular weight: 24 kDa). |
Target
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RelevanceMIS12 is part of a complex that plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly.
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Cellular localizationNucleus. Associated with the kinetochore.
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Database links
- Entrez Gene: 79003 Human
- Omim: 609178 Human
- SwissProt: Q9H081 Human
- Unigene: 267194 Human
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Alternative names
- hMIS12 antibody
- homolog of yeast Mis12 antibody
- KNTC2AP antibody
see all
Images
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Immunoprecipitation - Anti-MIS12 antibody (ab70843)HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70843 at 1 µg/ml for WB, subsequent to being used for IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds. -
Western blot - Anti-MIS12 antibody (ab70843)All lanes : Anti-MIS12 antibody (ab70843) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 34 kDa (why is the actual band size different from the predicted?)
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIS12 antibody (ab70843)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling MIS12 with ab70843 at 1/1000 (1µg/ml). Detection: DAB. -
Immunocytochemistry/ Immunofluorescence - Anti-MIS12 antibody (ab70843)This image is courtesy of an abreview by Eleni Petsalaki.ab70843 staining MIS12 in a human colon carcinoma cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour. An undiluted FITC-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Niikura Y et al. SGT1-HSP90 complex is required for CENP-A deposition at centromeres. Cell Cycle 16:1683-1694 (2017). Read more (PubMed: 28816574) »
- Zhou H et al. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun 7:10151 (2016). Read more (PubMed: 26743940) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"