Product nameAnti-MIS12 antibody
See all MIS12 primary antibodies
DescriptionRabbit polyclonal to MIS12
Tested applicationsSuitable for: IP, IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan, Bat
Synthetic peptide corresponding to a region between residue 150 and the C-terminus (residue 205) of human MIS12. (NP_076944.1)
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 - 5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000 - 1/2500. Detects a band of approximately 34 kDa (predicted molecular weight: 24 kDa).|
RelevanceMIS12 is part of a complex that plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly.
Cellular localizationNucleus. Associated with the kinetochore.
- hMIS12 antibody
- homolog of yeast Mis12 antibody
- KNTC2AP antibody
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70843 at 1 µg/ml for WB, subsequent to being used for IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-MIS12 antibody (ab70843) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling MIS12 with ab70843 at 1/1000 (1µg/ml). Detection: DAB.
ab70843 staining MIS12 in a human colon carcinoma cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour. An undiluted FITC-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
This product has been referenced in:
- Petsalaki E et al. Chmp4c is required for stable kinetochore-microtubule attachments. Chromosoma N/A:N/A (2018). Read more (PubMed: 29968190) »
- Niikura Y et al. SGT1-HSP90 complex is required for CENP-A deposition at centromeres. Cell Cycle 16:1683-1694 (2017). Read more (PubMed: 28816574) »