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    mis12-antibody-ab70843.pdf

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Epigenetics and Nuclear Signaling Chromosome Structure Centromeres
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Anti-MIS12 antibody (ab70843)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (5)

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Abpromise

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Find out more.

Immunoprecipitation - Anti-MIS12 antibody (ab70843)
  • Western blot - Anti-MIS12 antibody (ab70843)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIS12 antibody (ab70843)

Key features and details

  • Rabbit polyclonal to MIS12
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-MIS12 antibody
    See all MIS12 primary antibodies
  • Description

    Rabbit polyclonal to MIS12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residue 150 and the C-terminus (residue 205) of human MIS12. (NP_076944.1)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Centromeres
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Centromere
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Centromere

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

Our Abpromise guarantee covers the use of ab70843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 - 5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/2500. Detects a band of approximately 34 kDa (predicted molecular weight: 24 kDa).

Target

  • Relevance

    MIS12 is part of a complex that plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly.
  • Cellular localization

    Nucleus. Associated with the kinetochore.
  • Database links

    • Entrez Gene: 79003 Human
    • Omim: 609178 Human
    • SwissProt: Q9H081 Human
    • Unigene: 267194 Human
    • Alternative names

      • hMIS12 antibody
      • homolog of yeast Mis12 antibody
      • KNTC2AP antibody
      • MIND kinetochore complex component, homolog (yeast) antibody
      • MIS12 homolog (yeast) antibody
      • MIS12 homolog antibody
      • MIS12 MIND kinetochore complex component homolog (yeast) antibody
      • MTW 1 antibody
      • MTW1 antibody
      • Protein MIS12 homolog antibody
      see all

    Images

    • Immunoprecipitation - Anti-MIS12 antibody (ab70843)
      Immunoprecipitation - Anti-MIS12 antibody (ab70843)
      HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70843 at 1 µg/ml for WB, subsequent to being used for IP at 3 µg/mg lysate.
      Detection: Chemiluminescence with an exposure time of 30 seconds.
    • Western blot - Anti-MIS12 antibody (ab70843)
      Western blot - Anti-MIS12 antibody (ab70843)
      All lanes : Anti-MIS12 antibody (ab70843) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 24 kDa
      Observed band size: 34 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIS12 antibody (ab70843)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIS12 antibody (ab70843)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling MIS12 with ab70843 at 1/1000 (1µg/ml). Detection: DAB.

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab70843? Please let us know so that we can cite the reference in this datasheet.

    ab70843 has been referenced in 5 publications.

    • Petsalaki E  et al. Chmp4c is required for stable kinetochore-microtubule attachments. Chromosoma N/A:N/A (2018). PubMed: 29968190
    • Niikura Y  et al. SGT1-HSP90 complex is required for CENP-A deposition at centromeres. Cell Cycle 16:1683-1694 (2017). PubMed: 28816574
    • Zhou H  et al. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun 7:10151 (2016). PubMed: 26743940
    • Milev MP  et al. TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment. J Cell Biol 209:221-34 (2015). WB, ICC/IF ; Human . PubMed: 25918224
    • Petrovic A  et al. The MIS12 complex is a protein interaction hub for outer kinetochore assembly. J Cell Biol 190:835-52 (2010). WB ; Human . PubMed: 20819937

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MIS12 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Colon carcinoma)
    Permeabilization
    No
    Specification
    Colon carcinoma
    Fixative
    Methanol
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Jan 29 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MIS12 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    No
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 25 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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