Anti-MIS12 antibody (ab70843)
Key features and details
- Rabbit polyclonal to MIS12
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MIS12 antibody -
Description
Rabbit polyclonal to MIS12 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan, Bat -
Immunogen
Synthetic peptide corresponding to Human MIS12 (C terminal).
Database link: Q9H081 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-5 - 5 µg/mg of lysate.
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IHC-P |
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 24 kDa).
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Notes |
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IP
Use at 2-5 - 5 µg/mg of lysate. |
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 24 kDa). |
Target
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Relevance
MIS12 is part of a complex that plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly. -
Cellular localization
Nucleus. Associated with the kinetochore. -
Database links
- Entrez Gene: 79003 Human
- Omim: 609178 Human
- SwissProt: Q9H081 Human
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Alternative names
- hMIS12 antibody
- homolog of yeast Mis12 antibody
- KNTC2AP antibody
see all
Images
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All lanes : Anti-MIS12 antibody (ab70843) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 24 kDaDetection: Chemiluminescence with an exposure time of 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MIS12 with ab70843 at 1/5000 (1µg/ml). Detection: DAB.
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HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70843 at 1 µg/ml for WB, subsequent to being used for IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab70843 has been referenced in 7 publications.
- Wu Z et al. METTL3 counteracts premature aging via m6A-dependent stabilization of MIS12 mRNA. Nucleic Acids Res 48:11083-11096 (2020). PubMed: 33035345
- Bai GY et al. Mis12 controls cyclin B1 stabilization via Cdc14B-mediated APC/CCdh1 regulation during meiotic G2/M transition in mouse oocytes. Development 147:N/A (2020). PubMed: 32341029
- Petsalaki E et al. Chmp4c is required for stable kinetochore-microtubule attachments. Chromosoma N/A:N/A (2018). PubMed: 29968190
- Niikura Y et al. SGT1-HSP90 complex is required for CENP-A deposition at centromeres. Cell Cycle 16:1683-1694 (2017). PubMed: 28816574
- Zhou H et al. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun 7:10151 (2016). PubMed: 26743940
- Milev MP et al. TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment. J Cell Biol 209:221-34 (2015). WB, ICC/IF ; Human . PubMed: 25918224
- Petrovic A et al. The MIS12 complex is a protein interaction hub for outer kinetochore assembly. J Cell Biol 190:835-52 (2010). WB ; Human . PubMed: 20819937