Key features and details
- Rabbit polyclonal to MIST-1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MIST-1 antibody
See all MIST-1 primary antibodies
DescriptionRabbit polyclonal to MIST-1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human MIST-1 (internal sequence). (18 amino acid peptide; NP_803238).
Database link: Q7RTS1
- WB: Rat small intestine tissue lysate.
Protein previously known as BHLHA15.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 20 kDa).|
FunctionPlays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3'.
Tissue specificityExpressed in brain, liver, spleen and skeletal muscle.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
DomainLacks a classic transcription activation domain and instead possesses an N-terminal region capable of inhibiting heterologous activators.
- Information by UniProt
- Basic helix loop helix domain containing class B 8 antibody
- Basic helix loop helix family member a15 antibody
- BHA15_HUMAN antibody
All lanes : Anti-MIST-1 antibody (ab180889) at 1 µg/ml
Lane 1 : Rat small intestine tissue lysate
Lane 2 : Rat small intestine tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 20 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
ab180889 has been referenced in 1 publication.
- Lee Y et al. MIST1 regulates SNAI1 and acts through the PTEN/AKT signaling axis to promote anoikisresistance in human melanoma cells. Exp Ther Med 16:695-703 (2018). PubMed: 30112032