Overview

  • Product name

  • Description

    Rabbit polyclonal to MIST-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MIST-1 (internal sequence). (18 amino acid peptide; NP_803238).
    Database link: Q7RTS1

  • Positive control

    • WB: Rat small intestine tissue lysate.
  • General notes

    Protein previously known as BHLHA15.

Properties

Applications

Our Abpromise guarantee covers the use of ab180889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 20 kDa).

Target

  • Function

    Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3'.
  • Tissue specificity

    Expressed in brain, liver, spleen and skeletal muscle.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Domain

    Lacks a classic transcription activation domain and instead possesses an N-terminal region capable of inhibiting heterologous activators.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Basic helix loop helix domain containing class B 8 antibody
    • Basic helix loop helix family member a15 antibody
    • BHA15_HUMAN antibody
    • Bhlha15 antibody
    • bHLHB8 antibody
    • Class A basic helix-loop-helix protein 15 antibody
    • Class B basic helix-loop-helix protein 8 antibody
    • Class II bHLH protein MIST1 antibody
    • intestine and stomach expression 1 antibody
    • MIST 1 antibody
    • MIST-1 antibody
    • MIST1 antibody
    • Muscle antibody
    • Muscle intestine and stomach expression 1 antibody
    • protein MIST1 antibody
    see all

Images

  • All lanes : Anti-MIST-1 antibody (ab180889) at 1 µg/ml

    Lane 1 : Rat small intestine tissue lysate
    Lane 2 : Rat small intestine tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 20 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Lee Y  et al. MIST1 regulates SNAI1 and acts through the PTEN/AKT signaling axis to promote anoikisresistance in human melanoma cells. Exp Ther Med 16:695-703 (2018). Read more (PubMed: 30112032) »
See 1 Publication for this product

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