Key features and details
- Rabbit polyclonal to MiTF
- Suitable for: IHC-Fr, ICC/IF
- Reacts with: Mouse, Chicken, Human, Xenopus laevis
- Isotype: IgG
Product nameAnti-MiTF antibody
See all MiTF primary antibodies
DescriptionRabbit polyclonal to MiTF
Tested applicationsSuitable for: IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Chicken, Human, Xenopus laevis
Recombinant full length protein (His-tag) corresponding to Human MiTF.
Database link: O75030
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Preservative: 0.05% Sodium azide
Constituent: 99% Whole serum
Concentration information loading...
ChIP Related Products
Our Abpromise guarantee covers the use of ab122982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/300 - 1/1000.|
FunctionTranscription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Tissue specificityIsoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
Involvement in diseaseDefects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.
Sequence similaritiesBelongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain.
modificationsPhosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.
- Information by UniProt
- BHLHE32 antibody
- bHLHe32 antibody
- Class E basic helix-loop-helix protein 32 antibody
Immunohistochemical analysis of RPE (Retinal Pigment Epithelium) in chicken embryo (stage 14) labelling MiTF with ab122982 at dilution of 1/300. Embryo was fixed with paraformaldehyde and embedded in OCT compound and sectioned with a cryostat at 8
μm. Alexa Fluor® 488 conjugated anti-rabbit IgG was used at the secondary antibody.
At stage 14, Mitif protein is detected throughout the RPE (Retinal Pigment Epithelium).
Immunofluorescence analysis of mouse primary melanocytes labelling MiTF with ab122982 at a dilution of 1/300 (right). Nuclei couterstained with DAPI (blue) (left).
ab122982 has been referenced in 7 publications.
- Guo Y et al. Modeling Retinitis Pigmentosa: Retinal Organoids Generated From the iPSCs of a Patient With the USH2A Mutation Show Early Developmental Abnormalities. Front Cell Neurosci 13:361 (2019). PubMed: 31481876
- Moon KH et al. Differential Expression of NF2 in Neuroepithelial Compartments Is Necessary for Mammalian Eye Development. Dev Cell 44:13-28.e3 (2018). PubMed: 29249622
- Geng Z et al. Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PLoS One 12:e0173575 (2017). PubMed: 28282420
- Kerosuo L & Bronner ME cMyc Regulates the Size of the Premigratory Neural Crest Stem Cell Pool. Cell Rep 17:2648-2659 (2016). PubMed: 27926868
- Tsukiji N et al. Mitf functions as an in ovo regulator for cell differentiation and proliferation during development of the chick RPE. Dev Biol 326:335-46 (2009). PubMed: 19100253
- Delmas V et al. Beta-catenin induces immortalization of melanocytes by suppressing p16INK4a expression and cooperates with N-Ras in melanoma development. Genes Dev 21:2923-35 (2007). PubMed: 18006687
- Osawa M et al. Molecular characterization of melanocyte stem cells in their niche. Development 132:5589-99 (2005). PubMed: 16314490