Key features and details
- Rabbit polyclonal to MiTF (phospho S73)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MiTF (phospho S73) antibody
See all MiTF primary antibodies
DescriptionRabbit polyclonal to MiTF (phospho S73)
SpecificityDetects endogenous levels of MiTF only when phosphorylated at serine 73.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic phosphopeptide (Human) from around the phosphorylation site of serine 73 (PNSPPM)
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Storage instructionsStore at -20°C. Stable for 12 months at -20°C
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab59201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Tissue specificityIsoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
Involvement in diseaseDefects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.
Sequence similaritiesBelongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain.
modificationsPhosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.
- Information by UniProt
- BHLHE32 antibody
- bHLHe32 antibody
- Class E basic helix-loop-helix protein 32 antibody
All lanes : Anti-MiTF (phospho S73) antibody (ab59201) at 1/500 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract with phosphopeptide
Predicted band size: 59 kDa
Observed band size: 59 kDa
ab59201 has been referenced in 2 publications.
- Lee B et al. Swertiajaponin inhibits skin pigmentation by dual mechanisms to suppress tyrosinase. Oncotarget 8:95530-95541 (2017). PubMed: 29221146
- Han N et al. Evaluation of Fibroblast Growth Factor Receptor 2 Expression, Heterogeneity and Clinical Significance in Gastric Cancer. Pathobiology 82:269-79 (2015). PubMed: 26516773