Anti-Mitochondria antibody [113-1] - BSA and Azide free (ab92824)
Key features and details
- Mouse monoclonal [113-1] to Mitochondria - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Mitochondria antibody [113-1] - BSA and Azide free
See all Mitochondria primary antibodies -
Description
Mouse monoclonal [113-1] to Mitochondria - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Human cell homogenate
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Positive control
- WB: HeLa cell lysate. ICC/IF: HeLa and U87 cells. IHC-P: Human breast cancer tissue. Flow Cyt: HepG2 cells.
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General notes
This antibody is an excellent marker for Human cells in xenographic model research.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
This antibody is an excellent marker for Human cells in xenographic model research. -
Clonality
Monoclonal -
Clone number
113-1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 60 kDa.
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IHC-P | (4) |
1/1000.
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ICC/IF | (3) |
1/800.
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Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000. Detects a band of approximately 60 kDa. |
IHC-P
1/1000. |
ICC/IF
1/800. |
Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
Mitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales". -
Cellular localization
Mitochondrial
Images
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Immunostained human cells in spleen and temporal artery (TA) xenograft sections demonstrate persistent human PBMC colonization at 28 days
Mitochondria in spleen (B) and temporal artery xenografts (E) were detected using ab92824 at 1/400 dilution in immunohistochemical analysis.
Magnification: 400X for spleen, 200X for TA grafts and 800X for insets.
(From Figure 2 of Chen et al)
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Anti-Mitochondria antibody [113-1] - BSA and Azide free (ab92824) at 1/1000 dilution + HeLa lysate
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ICC/IF image of ab92824 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92824, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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IHC image of ab92824 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92824, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Overlay histogram showing HepG2 cells stained with ab92824 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92824, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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ab92824 staining mitochondria in the Human U87 glioblastoma cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 10% NBF, permeabilized with Triton X-100 and blocked with 2% serum for 90 minutes at 22°C. Samples were incubated with primary antibody (1/1000 in 0.2% BSA + 2% NGS) for 15 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-mouse IgG1 polyclonal was used as the secondary antibody (1/500). Co stained with ActinGreen, 2 drops/ml PBS, 10 min and Hoechst 2ug/ml in H2O 10 min
Protocols
Datasheets and documents
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Datasheet download
References (77)
ab92824 has been referenced in 77 publications.
- Alexander JF et al. Targeting the Meningeal Compartment to Resolve Chemobrain and Neuropathy via Nasal Delivery of Functionalized Mitochondria. Adv Healthc Mater 11:e2102153 (2022). PubMed: 35007407
- Chen K et al. Human Decidual Mesenchymal Stem Cells Obtained From Early Pregnancy Improve Cardiac Revascularization Postinfarction by Activating Ornithine Metabolism. Front Cardiovasc Med 9:837780 (2022). PubMed: 35242829
- Chen G et al. Apelin-13 Pretreatment Promotes the Cardioprotective Effect of Mesenchymal Stem Cells against Myocardial Infarction by Improving Their Survival. Stem Cells Int 2022:3742678 (2022). PubMed: 35355588
- Tontsch-Grunt U et al. Therapeutic impact of BET inhibitor BI 894999 treatment: backtranslation from the clinic. Br J Cancer 127:577-586 (2022). PubMed: 35444289
- Peng SY et al. Aldosterone Suppresses Endothelial Mitochondria through Mineralocorticoid Receptor/Mitochondrial Reactive Oxygen Species Pathway. Biomedicines 10:N/A (2022). PubMed: 35625856