Overview

  • Product name
    Anti-Mitochondria antibody [SPM198], prediluted
    See all Mitochondria primary antibodies
  • Description
    Mouse monoclonal [SPM198] to Mitochondria, prediluted
  • Host species
    Mouse
  • Specificity
    This antibody recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Semi-purified mitochondrial preparation

  • Positive control
    • Liver
  • General notes


    ab17857 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in human cell or tissue preparations and can be used as a mitochondrial-marker in subcellular fractions. ab17857 also detects the organelles within mitochondria; characteristic ('spaghetti'-like) mitochchondrial staining pattern is observed in the cytoplasm of normal and malignant human cells.

Properties

  • Form
    Prediluted
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 0.605% Tris, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    ab17857 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in human cell or tissue preparations and can be used as a mitochondrial-marker in subcellular fractions. ab17857 also detects the organelles within mitochondria; characteristic ('spaghetti'-like) mitochchondrial staining pattern is observed in the cytoplasm of normal and malignant human cells.
  • Clonality
    Monoclonal
  • Clone number
    SPM198
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Ready-to-use for 30 min at RT.

Target

  • Relevance
    Mitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales".
  • Cellular localization
    Mitochondrial

Images

  • ab17857 detecting mitochondria in human liver (dilution of ab17857 =  ready-to-use for 30min at RT)

References

This product has been referenced in:
  • Pusiol T  et al. Value of imaging and aspiration cytology in the diagnosis of oncocytic carcinoma. Acta Otorhinolaryngol Ital 30:110-4 (2010). Read more (PubMed: 20559483) »
  • Lévesque MH  et al. Androgen receptor as a potential sign of prostate cancer metastasis. Prostate 69:1704-11 (2009). IHC-P ; Human . Read more (PubMed: 19670238) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

Regarding antibody ab17857 I am afraid we have not tested it with mouse samples, therefore the cross-reactivity with this species is not known.

We have other antibodies against Mitochondria validated in IHC-P, like ab80649, but this one is from mouse, so there is a likelihood that it will react with mouse tissue.

However ab92824 is specific for human mitochondria and does not react with rat or mouse, so it may be suitable for your application.

I hope this information was useful. If not, please do not hesitate to contact us back.

Read More

Answer

Thank you for your enquiry and interest. As the datasheets indicate these antibodies have been tested for cross-reaction with human but not data available for mouse. All of these three antibodies are raised in mouse, so high non-specific background could be a problem using mouse tissue sections in IHC. However, it has not been determined if ab3298, ab17857, ab79479 recognize murine mitochondrial proteins. If you need any further assistance in the future, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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