Question (75087) | Mitochondria Isolation Kit for Cultured Cells (ab110170)

Go to datasheet (ab110170)

Question

Our customer wonders if the final product of ab110170 (the isolated mitochondria) can be used in in vitro transcription "in organello". This customer also would like to know the expected yield of the isolation.
Thanks in advance for your assistance and reply.
Best Regards,

Answer

I can confirm that the mitochondria isolated using this kit can be used in other biological assays.
Please check protocol booklet page number 2 for expected yield based on cell line:
Sample / Starting Material / Expected Yield
MRC-5 / 4 – 150 mm plates / 0.5 mg*
HepG2 / 4 – 150 mm plates / 1.0 mg*
HDFN / 2 – 150 mm plates / 0.5 mg*